Low molecular weight polyethylenimine as a transgenic vector for tumor gene therapy. (February 2015)

• Record Type: Journal Article
• Authors: Lou, Z; Xing, H; Pan, H; Lou, F; Wang, K; Fang, Y; Han, W; Li, D
• Is Part Of: Biotechnic & histochemistry. Volume 90:Number 2(2015)
• Page Start: 140
• Page End: 145
• Publication Date: 2015-02
• Subjects: FGF -- gene delivery -- gene therapy -- polyethylenimine -- receptor -- tumor -- vector; Histochemistry -- Periodicals; Stains and staining (Microscopy) -- Periodicals; Biotechnology -- Periodicals; 572
• DOI: 10.3109/10520295.2014.965278
• Languages: English
• ISSNs: 1052-0295

Abstract

Abstract: We constructed a polymer composed of a series of small molecule polyethylenimine (PEI) using 4-arm polyethylenimine (PEG) as a core for tumor gene therapy. N, N'-carbonyldiimidazole and N-succinimidyl-3-(2-pyridyldithio) propionate were used as chemical connecting reagents to synthesize 4-arm PEG-PEI2000 and 4-arm PEG-PEI2000-MC11. Chemical characterization was performed using 1 H-NMR. The retardation effect of polymers on plasmid DNA was observed using electrophoretic mobility shift and MTT assays to test the toxicity of the polymers. The gene delivery capability of 4-arm PEG-PEI2000 and 4-arm PEG-PEI2000-MC11, and the effect of MC11 were determined by an in vitro gene delivery experiment with human hepatoma HepG2 cells. At a N:P ratio of 3, the 4-arm PEG-PEI2000 could retard successfully plasmid DNA with low toxicity. In experiments in vitro, when the N:P ratio was 30, the gene delivery efficiency of 4-arm PEG-PEI2000 in HepG2 cells was five times that of PEI2000; After connecting ligand MC11, however, the gene delivery efficiency was twice as great. Free MC11 effectively inhibited the gene delivery efficiency of the 4-arm PEG-PEI2000-MC11. Four-arm PEG-PEI2000 has low toxicity and high gene delivery efficiency, and is an effective gene delivery vector after linking ligand MC11.