Antibacterial properties and atomic resolution X-ray complex crystal structure of a ruthenocene conjugated β-lactam antibiotic. Issue 28 (10th March 2015)

• Record Type: Journal Article
• Authors: Lewandowski, Eric M.; Skiba, Joanna; Torelli, Nicholas J.; Rajnisz, Aleksandra; Solecka, Jolanta; Kowalski, Konrad; Chen, Yu
• Is Part Of: Chemical communications. Volume 51:Issue 28(2015)
• Page Start: 6186
• Page End: 6189
• Publication Date: 2015-03-10
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c5cc00904a
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Crystal structure of β-lactam product complex with CTX-M-14 E166A mutant sheds light on β-lactamase catalysis and ruthenocene binding by proteins. Abstract : We have determined a 1.18 Å resolution X-ray crystal structure of a novel ruthenocenyle-6-aminopenicillinic acid in complex with CTX-M β-lactamase, showing unprecedented details of interactions between ruthenocene and protein. As the first product complex with an intact catalytic serine, the structure also offers insights into β-lactamase catalysis and inhibitor design.