Direct sequencing of a disulfide-linked peptide with electrospray ionization tandem mass spectrometry. Issue 8 (6th March 2015)

• Record Type: Journal Article
• Authors: Li, Gongyu; Pei, Jiying; Yin, Yue; Huang, Guangming
• Is Part Of: Analyst. Volume 140:Issue 8(2015)
• Page Start: 2623
• Page End: 2627
• Publication Date: 2015-03-06
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c5an00011d
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : Enhanced corona discharge was employed for in-spray dissociation of disulfide bonds, facilitating disulfide-containing peptide sequencing with ESI-MS/MS. Abstract : Dissociation of disulfide is normally mandatory prior to disulfide peptide sequencing via electrospray ionization collision induced dissociation mass spectrometry (ESI-CID-MS). Herein, a facile method for directly sequencing intact disulfide peptides was proposed. The basic principles involved were electrolyte-enhanced corona discharge in ESI and the following oxidative cleavage reaction.