Effect of bone marrow-derived stem cells and bone morphogenetic protein-2 on treatment of osteoradionecrosis in a rat model. Issue 8 (October 2015)

• Record Type: Journal Article
• Authors: Jin, Im Geon; Kim, Jin Ho; Wu, Hong-Gyun; Kim, Sook Kyoung; Park, Yongdoo; Hwang, Soon Jung
• Is Part Of: Journal of cranio-maxillofacial surgery. Volume 43:Issue 8(2015:Dec.)
• Page Start: 1478
• Page End: 1486
• Publication Date: 2015-10
• Subjects: Bone marrow-derived stem cell -- Bone morphogenetic protein-2 -- Osteoradionecrosis; Skull -- Surgery -- Periodicals; Maxilla -- Surgery -- Periodicals; Face -- Surgery -- Periodicals; Surgery, Plastic -- Periodicals; Maxilla -- surgery -- Periodicals; Face -- surgery -- Periodicals; Skull -- surgery -- Periodicals; Oral Surgical Procedures -- Periodicals; Reconstructive Surgical Procedures -- Periodicals; Surgery, Plastic -- Periodicals; Surgery, Oral -- Periodicals; Electronic journals; 617.514
• DOI: 10.1016/j.jcms.2015.06.035
• Languages: English
• ISSNs: 1010-5182

Abstract

Abstract: Purpose: We investigated the effect of rat mesenchymal stem cells (rMSCs) and bone morphogenetic protein-2 (BMP-2) on the osseous healing of osteoradionecrosis in the rat mandible, depending on application time. Material and methods: One week after irradiation (30 Gy) on the right mandible, all molar teeth were extracted. In Group 1 (n = 25), rMSCs and/or BMP-2 carried with hydrogel were applied immediately after surgery. In Group 2 (n = 25), application was done 4 weeks after surgery, after the occurrence of osteoradionecrosis. No hydrogel (n = 5), hydrogel alone (n = 5), hydrogel with 2 × 10 4 rMSCs (n = 5), hydrogel with 10 μg of BMP-2 (n = 5), or hydrogel with 10 μg of BMP-2 and 2 × 10 4 rMSCs (n = 5) was applied. Micro-CT data of bone healing were collected at 4 weeks post-application. Results: In Group 1, BMP-2 was effective in increasing both bone volume (BV) and bone mineral density (BMD), whereas rMSCs were not. In Group 2, the combined application of rMSCs and BMP-2 significantly increased BMD and BV compared to values in the groups without hydrogel and with hydrogel alone. Conclusion: Osseous healing after post-irradiation trauma in rats was enhanced immediately after dentoalveolar trauma by application of BMP-2, whereas the combined application of rMSCs and BMP-2 was most effective after osteoradionecrosis occurred.