Degradation of azo dye methyl red by Saccharomyces cerevisiae ATCC 9763. (November 2017)

• Record Type: Journal Article
• Authors: Vatandoostarani, Simin; Bagheri Lotfabad, Tayebe; Heidarinasab, Amir; Yaghmaei, Soheila
• Is Part Of: International biodeterioration & biodegradation. Volume 125(2017)
• Page Start: 62
• Page End: 72
• Publication Date: 2017-11
• Subjects: Azo bond -- Methyl red -- Saccharomyces cerevisiae -- Biodegradation; Biodegradation -- Periodicals; Bioremediation -- Periodicals; Biodegradation -- Periodicals; Biodégradation -- Périodiques; Biorestauration -- Périodiques; Electronic journals; 620.11223
• DOI: 10.1016/j.ibiod.2017.08.009
• Languages: English
• ISSNs: 0964-8305

Abstract

Abstract: Azo dyes are common dye groups widely used in different industries. Industrial effluents which contain these dyes pervade water resources and have various known and unknown physiological effects on living organisms. In this study, methyl red (MR) was proposed as a model azo dye to examine the capability of Saccharomyces cerevisiae ATCC 9763 for cleavage of the azo bond leading to decolourization. The spectrophotometry method showed that complete decolourization occurred statically during ≃12 h and this time reduced to several minutes less than 10 min where cell mass increased. Thin layer chromatography (TLC), mass spectroscopy and Fourier transform infrared (FTIR) confirmed degradation of methyl red to two constituents, N, N-dimethyl-p-phenylene diamine (DMPD) and 2-Aminobenzoic acid (2-ABA). In addition, a molecular mass of 122 observed in mass analysis results does not correspond with any compounds shown in the biodegradation pathway of methyl red developed by previous researchers; therefore, further study is needed to explain this variance. Graphical abstract: Highlights: Saccharomyces cerevisiae ATCC 9763 as a GRAS microorganism degraded methyl red. Static decolourization process was completed after several minutes. Methyl red in pure distilled water could be completely decolourized. Decolourization system was easily handled and operated. An environmentally friendly approach was developed with S. cerevisiae for azo bond degradation.