CD4+ T cell surface alpha enolase is lower in older adults. (December 2015)

• Record Type: Journal Article
• Authors: Bennett, Stuart J.; Augustyniak, Edyta M.; Dunston, Christopher R.; Brown, Richard A.; Shantsila, Eduard; Lip, Gregory Y.H.; Torrao, Rita D.C.; Pararasa, Chathyan; Remtulla, Ali H.; Ladouce, Romain; Friguet, Bertrand; Griffiths, Helen R.
• Is Part Of: Mechanisms of ageing and development. Volume 152(2015)
• Page Start: 56
• Page End: 62
• Publication Date: 2015-12
• Subjects: Enolase -- CD4+T cell -- Plasma membrane proteomics -- 2D gel electrophoresis -- Phenotype; Aging -- Periodicals; Developmental biology -- Periodicals; Aging -- Periodicals; Developmental Biology -- Periodicals; Vieillissement -- Périodiques; Biologie du développement -- Périodiques; Aging; Developmental biology; Periodicals; 612.67
• DOI: 10.1016/j.mad.2015.09.005
• Languages: English
• ISSNs: 0047-6374

Abstract

Highlights: α-Enolase is lower on the surface of CD4 + T cell in healthy older adults. Cardiovascular disease patients express less CD4 + T cell α-enolase than age-matched controls. A hypothesis-free proteomic discovery approach has been adopted successfully to study human ageing. Membrane protein enrichment has been developed to reduce bias towards cytosolic protein discovery. Abstract: To identify novel cell ageing markers in order to gain insight into ageing mechanisms, we adopted membrane enrichment and comparison of the CD4 + T cell membrane proteome (purified by cell surface labelling using Sulfo-NHS-SS-Biotin reagent) between healthy young ( n = 9, 20–25 years) and older ( n = 10; 50–70 years) male adults. Following two-dimensional gel electrophoresis (2DE) to separate pooled membrane proteins in triplicates, the identity of protein spots with age-dependent differences ( p < 0.05 and >1.4 fold difference) was determined using liquid chromatography–mass spectrometry (LC–MS/MS). Seventeen protein spot density differences (ten increased and seven decreased in the older adult group) were observed between young and older adults. From spot intensity analysis, CD4 + T cell surface α-enolase was decreased in expression by 1.5 fold in the older age group; this was verified by flow cytometry ( n = 22) and qPCR with significantly lower expression of cellular α-enolase mRNA and protein compared to young adult CD4 + T cells ( p < 0.05). In an independent age-matched case-control study, lower CD4 + T cell surface α-enolase expression was observed in age-matched patients with cardiovascular disease ( p < 0.05). An immune-modulatory role has been proposed for surface α-enolase and our findings of decreased expression suggest that deficits in surface α-enolase merit investigation in the context of immune dysfunction during ageing and vascular disease.