Antifungal activities of secondary metabolites isolated from liquid fermentations of Stereum hirsutum (Sh134-11) against Botrytis cinerea (grey mould agent). (November 2017)

• Record Type: Journal Article
• Authors: Aqueveque, Pedro; Céspedes, Carlos L.; Becerra, José; Aranda, Mario; Sterner, Olov
• Is Part Of: Food and chemical toxicology. Volume 109:Part 2(2017)
• Page Start: 1048
• Page End: 1054
• Publication Date: 2017-11
• Subjects: Stereum -- Submerged cultures -- Antifungal -- Botrytis -- Sterenin D -- Chilean fungi; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2017.05.036
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Extracts obtained from liquid mycelial fermentations of the Chilean fungus Stereum hirsutum (Sh134-11) showed antifungal activity against Botrytis cinerea. Two types of extracts were obtained: EtOAc-extract (liquid phase) and MeOH-extract (mycelial phase). Plate diffusion assay showed that EtOAc-extracts were more active than MeOH-extracts. A large-scale fermentation of Sh134-11 and chromatographic methods allowed to isolated four compounds: MS-3, Vibralactone, Vibralactone B and Sterenin D. Only Sterenin D showed antifungal activity against B. cinerea in the tests performed. Effects on the mycelial growth of B. cinerea showed that Sterenin D showed inhibition at 1000–2000 μg/mL reaching 67% and 76% respectively. Sterenin D was more effective to control the sporogenesis, inhibiting in 96% the sporulation at 500 μg/mL. Assays showed that Sterenin D exhibited a minimal fungicidal concentration (MFC) of 50 μg/mL and minimal inhibitory concentration (MIC) at 20 μg/mL. Our study indicated that submerged fermentations of Chilean S. hirsutum (Sh134-11) produced extracts with antifungal activity and Sterenin D is responsible for this activity, which could be used as possible biofungicides alternative to synthetic fungicides. Graphical abstract: Highlights: Large-scale fermentation of Chilean fungus S. hirsutum (Sh-134-11) produced active extracts against B. cinerea. Bioassay-guided fractionation allowed to identify 4 compounds: MS-3, Vibralactone, Vibralactone B and Sterenin D. Only sterenin D showed antifungal activity inhibiting mycelial growth and sporogenesis of B. cinerea. Sterenin D showed MIC and MFC values at 10 and 50 μg/mL against B. cinerea respectively.