Analysis of endocrine disruption effect of Roundup® in adrenal gland of male rats. (2015)

• Record Type: Journal Article
• Authors: Pandey, Aparamita; Rudraiah, Medhamurthy
• Is Part Of: Toxicology reports. Volume 2(2015)
• Page Start: 1075
• Page End: 1085
• Publication Date: 2015
• Subjects: EDC endocrine disrupting chemical -- LD50 lethal dose, 50% -- Ldlr low density lipoprotein receptor -- Sr-b1 scavenger receptor class B member 1 -- Hmgcs 3-hydroxy-3-methylglutaryl-coenzyme A synthase -- Hmgcr 3-hydroxy-3-methylglutaryl-CoA reductase -- Hsl hormone-sensitive lipase -- StAR steroidogenic acute regulatory protein -- Creb cAMP response element-binding protein -- ACTH adrenocorticotropic hormone -- Mc2r melanocortin-2 receptor -- PKA protein kinase A -- cAMP cyclic adenosine monophosphate -- L:D cycle light–dark cycle -- RIA radioimmunoassay -- ELISA enzyme-linked immunosorbent assay -- EIA enzyme Immunoassay -- qPCR quantitative real-time PCR -- DPX distrene, plasticiser, xylene -- DAPI 4′, 6-diamidino-2-phenylindole -- RIPA buffer radioimmunoprecipitation assay buffer -- SDS PAGE sodium dodecyl sulfate polyacrylamide gel electrophoresis -- EDTA ethylenediaminetetraacetate -- EGTA ethylene glycol tetraacetate -- β ME beta mercaptoethanol -- PBS phosphate buffer saline -- TUNEL terminal deoxynucleotidyl transferase dUTP nick end labeling -- TdT terminal deoxynucleotidyl transferase -- SD standard deviation; Endocrine disruptor -- Glyphosate -- Steroidogenesis -- StAR -- Adrenal gland -- Rat; Toxicology -- Periodicals; Clinical toxicology -- Periodicals; Drug-Related Side Effects and Adverse Reactions; Hazardous Substances; Poisoning; Toxicology; Electronic journals; Periodicals; Periodicals; 571.9505
• DOI: 10.1016/j.toxrep.2015.07.021
• Languages: English
• ISSNs: 2214-7500

Abstract

Abstract: The effect of Roundup ® on adrenal gland steroidogenesis and signaling pathway associated with steroid production was investigated. Doses of 10, 50, 100 and 250 mg/kg bw/d Roundup ® were administered for two weeks to adult male rats. The 10 mg/kg bw/d dose which reduced circulatory corticosterone levels, but did not change food consumption and body weight, was selected for further study. The expression of cholesterol receptor (low density lipoprotein receptor), de novo cholesterol synthesis enzyme (3-hydroxy-3-methylglutaryl-coenzyme A synthase), hormone-sensitive lipase, steroidogenic acute regulatory protein (StAR) mRNA and phosphorylated form was decreased. Adrenocorticotropic hormone receptor (ACTH), melanocortin-2 receptor, expression was not changed but circulatory ACTH levels and adrenal cortex protein kinase A (PKA) activity were reduced. Surprisingly, exogenous ACTH treatment rescued steroidogenesis in Roundup ® -treated animals. Apoptosis was evident at 250 mg/kg bw/d, but not at 10 mg/kg bw/d dose. These results suggest that Roundup ® may be inhibitory to hypothalamic–pituitary axis leading to reduction in cyclic adenosine monophosphate (cAMP)/PKA pathway, StAR phosphorylation and corticosterone synthesis in the adrenal tissue.