Point-of-use Ultrafast Single-step Detection of Food Contaminants: A Novel Microfluidic Fluorescence-based Immunoassay with Integrated Photodetection. (2016)

• Record Type: Journal Article
• Authors: Soares, R.R.G.; Santos, D.R.; Pinto, I.F.; Azevedo, A.M.; Chu, V.; Aires-Barros, M.R.; Conde, J.P.
• Is Part Of: Procedia engineering. Volume 168(2016)
• Page Start: 329
• Page End: 332
• Publication Date: 2016
• Subjects: ultrafast -- mycotoxin -- beads -- fluorescence -- photodiodes -- real-time -- microfluidics; Engineering -- Congresses; Engineering -- Periodicals; Engineering; Conference proceedings; Periodicals; 620.005
• DOI: 10.1016/j.proeng.2016.11.208
• Languages: English
• ISSNs: 1877-7058

Abstract

Abstract: The assurance of strict food safety standards is a major challenge in modern society. Mycotoxins, in particular, are produced by fungi during production, storage or transport, posing serious health concerns to humans and animals. Aiming at developing a point-of-use tool for the rapid detection of mycotoxins at the required regulatory levels, we report a microfluidic ultrafast and single-step fluorescence-based immunoassay, using minimal amounts of reagents. This assay was integrated with 200×200 μm thin-film a-Si:H photodiodes towards a compact detection system. Aflatoxin B1 was detected at 1 ng/mL after 2 min assay time, among the fastest reported in the literature.