Vaccination with recombinant L7/L12-truncated Omp31 protein induces protection against Brucella infection in BALB/c mice. Issue 2 (June 2015)

• Record Type: Journal Article
• Authors: Golshani, Maryam; Rafati, Sima; Dashti, Amir; Gholami, Elham; Siadat, Seyed Davar; Oloomi, Mana; Jafari, Anis; Bouzari, Saeid
• Is Part Of: Molecular immunology. Volume 65:Issue 2(2015:Jun.)
• Page Start: 287
• Page End: 292
• Publication Date: 2015-06
• Subjects: Brucella melitensis -- Brucella abortus -- Fusion protein -- Human vaccine -- L7/L12 -- Truncated Omp31; Immunochemistry -- Periodicals; Molecular biology -- Periodicals; Immunochemistry -- Periodicals; Allergy and Immunology -- Periodicals; Molecular Biology -- Periodicals; Immunochimie -- Périodiques; Biologie moléculaire -- Périodiques; Immunochemistry; Molecular biology; Periodicals; Electronic journals; 571.96
• DOI: 10.1016/j.molimm.2015.01.009
• Languages: English
• ISSNs: 0161-5890

Abstract

Highlights: We evaluate the immunogenicity and protective efficiency of the rL7/L12-TOmp31 against Brucella . rL7/L12-TOmp31 immunizations induce a vigorous humoral response. rL7/L12-TOmp31 immunization induces a Th1 cellular response. rL7/L12-TOmp31 immunization induces protection against B. melitensis and B. abortus challenge. rL7/L12-TOmp31 could be a potential antigen candidate for development of new subunit vaccines against B. melitensis and B. abortus . Abstract: Brucellosis is the most common bacterial zoonotic disease worldwide and no vaccine is available for the prevention of human brucellosis. In humans, brucellosis is mostly caused by Brucella melitensis and Brucella abortus . The Outer membrane protein 31 (Omp31) and L7/L12 are immunodominant and protective antigens conserved in human Brucella pathogens. In the present study, we evaluated the humoral and cellular immune responses induced by a fusion protein designed based on the Truncated form of Omp31 (TOmp31) and L7-L12 antigens. Vaccination of BALB/c mice with the recombinant fusion protein (rL7/L12-TOmp31) provided the significant protection level against B. melitensis and B. abortus challenge. Moreover, rL7/L12-TOmp31 elicited a strong specific IgG response (higher IgG2a titers) and significant IFN-γ/IL2 production and T-cell proliferation was also observed. The T helper1 (Th1) oriented response persisted for 12 weeks after the first immunization. The rL7/L12-TOmp31 could be a new potential antigen candidate for the development of a subunit vaccine against B. melitensis and B. abortus .