A microfluidic cell culture system for monitoring of sequential changes in endothelial cells after heat stress. Issue 2 (August 2015)

• Record Type: Journal Article
• Authors: Tazawa, Hidekatsu; Sato, Kenjiro; Tsutiya, Atsuhiro; Tokeshi, Manabu; Ohtani-Kaneko, Ritsuko
• Is Part Of: Thrombosis research. Volume 136:Issue 2(2015)
• Page Start: 328
• Page End: 334
• Publication Date: 2015-08
• Subjects: Microfluidic cell culture system -- Microchip -- Endothelial cells -- Heat-shock response -- PAI-1; Thrombosis -- Periodicals; 616.135
• DOI: 10.1016/j.thromres.2015.05.008
• Languages: English
• ISSNs: 0049-3848

Abstract

Abstract: Endothelial damage induced by a highly elevated body temperature is crucial in some diseases including viral hemorrhagic fevers. Here, we report the heat-induced sequential changes of endothelial cells under shear stress, which were determined with a microfluidic culture system. Although live cell imaging showed only minor changes in the appearance of heat-treated cells, Hsp70 mRNA expression analysis demonstrated that the endothelial cells in channels of the system responded well to heat treatment. F-actin staining also revealed clear changes in the bundles of actin filaments after heat treatment. Well-organized bundles of actin filaments in control cells disappeared in heat-treated cells cultured in the channel. Furthermore, the system enabled detection of sequential changes in plasminogen activator inhibitor-1 (PAI-1) secretion from endothelial cells. PAI-1 concentration in the effluent solution was significantly elevated for the first 15 min after initiation of heat treatment, and then decreased subsequently. This study provides fundamental information on heat-induced endothelial changes under shear stress and introduces a potent tool for analyzing endothelial secretions. Highlights: We examine the heat-induced sequential changes of endothelial cells under shear stress with a microfluidic culture system. The endothelial cells in channels of the system responded well to heat treatment. The bundles of F-actin filaments collapse after heat treatment. We can detect sequential changes in PAI-1 secretion from endothelial cells after heat treatment.