Contrasting effects of G1.2/G2.2 and SOF1/SOF2 embryo culture media on pre- and post-implantation development of non-transgenic and transgenic cloned goat embryos. Issue 3 (September 2015)

• Record Type: Journal Article
• Authors: Hosseini, Sayed Morteza; Hajian, Mehdi; Ostadhosseini, Somayyeh; Forouzanfar, Mohsen; Abedi, Parvaneh; Jafarpour, Farnoosh; Gourabi, Hamid; Shahverdi, Abdol Hossein; Vosough, Ahmad; Ghanaie, Hamid Reza; Nasr-Esfahani, Mohammad Hossein
• Is Part Of: Reproductive biomedicine online. Volume 31:Issue 3(2015)
• Page Start: 372
• Page End: 383
• Publication Date: 2015-09
• Subjects: embryo development -- G1.2/G2.2 -- gene expression -- goat -- nuclear transfer -- SOF1/SOF2; Human reproductive technology -- Periodicals; Human embryo -- Periodicals; Reproduction -- Periodicals; 616.692
• DOI: 10.1016/j.rbmo.2015.06.008
• Languages: English
• ISSNs: 1472-6483

Abstract

Highlights: Significantly higher in-vitro development of goat cloned embryos in G1.2/G2.2 vs. SOF1/SOF2. Significantly higher in-vivo development of goat cloned embryos in SOF1/SOF2 vs. G1.2/G2.2. Up-regulation of WNT, FGF and TGFβ signaling pathways in goat cloned embryos cultured in SOF1/SOF2 compared with those cultured in G1.2/G2.2. Abstract: This study compared the efficiency of two embryo culture media (SOF1/SOF2 and G1.2/G2.2) for pre- and post-implantation development of somatic cell nuclear transfer goat embryos derived from non-transgenic and transgenic (for htPA and hrcfIX genes) fibroblasts. Despite similar cleavage rates, G1.2/G2.2 supported significantly higher blastocyst development than SOF1/SOF2 (30–35% versus 21%; P < 0.05), irrespective of cell transgenesis. However, following embryo transfer, pregnancy outcomes (establishment, full-term development and live birth) were all significantly higher ( P < 0.05) for embryos developed in SOF1/SOF2 versus G1.2/G2.2. Gene expression profiling of 17 developmentally important genes revealed that: (i) SOX2, FOXD3, IFNT, FZD, FGFR4, ERK1, GCN5, PCAF, BMPR1, SMAD5, ALK4, CDC25 and LIFR were significantly induced in blastocysts developed in SOF1/SOF2 but not G1.2/G2.2; (ii) OCT4, CTNNB and CDX2 were similarly expressed in both groups; and (iii) AKT was significantly higher in G1.2/G2.2 than SOF1/SOF2 ( P < 0.05). Following IVF, although blastocyst development in G1.2/G2.2 was significantly higher than SOF1/SOF2 counterparts, the majority of assessed genes were similarly expressed in blastocysts developed in both groups. It was concluded that the long-term programming effects of embryo culture medium and/or embryo production method may irreversibly affect post-implantation development of cloned embryos through defined molecular pathways.