RNA over-editing of BLCAP contributes to hepatocarcinogenesis identified by whole-genome and transcriptome sequencing. Issue 2 (28th February 2015)

• Record Type: Journal Article
• Authors: Hu, Xueda; Wan, Shengqing; Ou, Ying; Zhou, Boping; Zhu, Jialou; Yi, Xin; Guan, Yanfang; Jia, Wenlong; Liu, Xing; Wang, Qiudao; Qi, Yao; Yuan, Qing; Huang, Wanqiu; Liao, Weijia; Wang, Yun; Zhang, Qinghua; Xiao, Huasheng; Chen, Xinchun; Huang, Jian
• Is Part Of: Cancer letters. Volume 357:Issue 2(2015)
• Page Start: 510
• Page End: 519
• Publication Date: 2015-02-28
• Subjects: RNA over-editing -- BLCAP gene -- Hepatocarcinogenesis -- Whole-genome and transcriptome sequencing; Cancer -- Periodicals; Neoplasms -- Periodicals; Cancer -- Périodiques; Electronic journals; 616.994
• DOI: 10.1016/j.canlet.2014.12.006
• Languages: English
• ISSNs: 0304-3835

Abstract

Highlights: There are widespread RNA editing events in the transcriptome of human liver tissue using NGS technology. There is differential expression of RNA editing genes in HCC compared to non tumor liver. BLCAP RNA over-editing contributes to hepatocarcinogenesis. BLCAP RNA editing can activate the Akt/mTOR signaling pathway by affecting the phosphorylation levels. Abstract: Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide, although the treatment of this disease has changed little in recent decades because most of the genetic events that initiate this disease remain unknown. To better understand HCC pathogenesis at the molecular level and to uncover novel tumor-initiating events, we integrated RNA-seq and DNA-seq data derived from two pairs of HCC tissues. We found that BLCAP is novel editing gene in HCC and has over-editing expression in 40.1% HCCs compared to adjacent liver tissues. We then used RNA interference and gene transfection to assess the roles of BLCAP RNA editing in tumor proliferation. Our results showed that compared to the wild-type BLCAP gene, the RNA-edited BLCAP gene may stably promote cell proliferation (including cell growth, colony formation in vitro, and tumorigenicity in vivo) by enhancing the phosphorylation of AKT, mTOR, and MDM2 and inhibiting the phosphorylation of TP53. Our current results suggest that the RNA over-editing of BLCAP gene may serve as a novel potential driver in advanced HCC through activating AKT/mTOR signal pathway.