In vitro evaluation of the inhibitory effect of canine serum, canine fresh frozen plasma, freeze–thaw‐cycled plasma, and Solcoseryl™ on matrix metalloproteinases 2 and 9. (18th August 2014)

• Record Type: Journal Article
• Authors: Chow, Derek W. Y.; Chau, Ying; Yeung, Wai Kit; Westermeyer, Hans D.
• Is Part Of: Veterinary ophthalmology. Volume 18:Number 3(2015)
• Page Start: 229
• Page End: 233
• Publication Date: 2014-08-18
• Subjects: hemodialysate -- keratomalacia -- matrix metalloproteinases -- plasma -- serum -- Solcoseryl™; Veterinary ophthalmology -- Periodicals; 636.08977
• DOI: 10.1111/vop.12204
• Languages: English
• ISSNs: 1463-5216

Abstract

Abstract: Objective: Compare the efficacy of canine serum, fresh frozen plasma (FFP), freeze–thaw‐cycled plasma (FTCP), and Solcoseryl ™ at inhibiting matrix metalloproteinases (MMP) 2 and 9 in vitro . Procedure: Matrix metalloproteinases 2 and 9 activity in the presence of serum, FFP, FTCP, or Solcoseryl ™ was assayed using a commercially available fluorogenic gelatinase activity kit. Results: Matrix metalloproteinases 2 activity in the presence of serum, FFP, FTCP, and Solcoseryl ™ was 20.84%, 5.76%, 8.10%, and 83.03%, respectively of uninhibited MMP 2 activity. MMP 9 activity in the presence of serum, FFP, FTCP, and Solcoseryl ™ was 57.36%, 58.35%, 49.35%, and −8.69%, respectively of uninhibited MMP 9 activity. Conclusion: Serum, FFP, and FTCP exhibit similar levels of MMP 2 and 9 inhibitions. Solcoseryl ™ causes minimal MMP 2 inhibition, but profound MMP 9 inhibition.