An isoflavone compound daidzein elicits myoblast differentiation and myotube growth. (November 2017)

• Record Type: Journal Article
• Authors: Lee, Sang-Jin; Vuong, Tuan Anh; Go, Ga-Yeon; Song, Yoo-Jin; Lee, Seulah; Lee, Soo-Yeon; Kim, Shin-Woong; Lee, Jaecheol; Kim, Yong Kee; Seo, Dong-Wan; Kim, Ki Hyun; Kang, Jong-Sun; Bae, Gyu-Un
• Is Part Of: Journal of functional foods. Volume 38:Part A(2017)
• Page Start: 438
• Page End: 446
• Publication Date: 2017-11
• Subjects: BS Ext Black soybean extract -- DEX dexamethasone -- DM differentiation medium -- DMEM Dulbecco modified Eagle's medium -- ER estrogen receptors -- ERE estrogen response element -- FBS fetal bovine serum -- GLUT4 glucose transporter 4 -- GM growth medium -- mTOR mammalian target of rapamycin -- MHC myosin heavy chain -- SDS-PAGE sodium dodecyl sulfatepolyacrylamide gel electrophoresis -- SRC-1 steroid hormone receptor coactivator -- Stx4 Syntaxin 4; Akt signaling -- Daidzein -- Muscle regeneration -- Muscular atrophy -- Myoblast differentiation -- MyoD; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2017.09.016
• Languages: English
• ISSNs: 1756-4646

Abstract

Abstract: Preservation of muscle stem cell function is critical for muscle homeostasis and the regenerative capacity of stem cells declines with age, leading to muscle loss. Previously, daidzein, a natural isoflavone from Leguminosae, has been shown to prevent TNF-α-induced muscle atrophy. However, the molecular mechanisms of daidzein in these processes are currently unknown. In this study, we demonstrate a positive effect of black soybean extract (BS Ext) and daidzein on myoblast differentiation and myotube growth. Daidzein prevents dexamethasone-induced myotube atrophy through Akt activation. The promyogenic effect of BS Ext and daidzein is mediated through two promyogenic kinases, Akt and p38MAPK that in turn activates a key myogenic transcription factor MyoD. Additionally, BS Ext and daidzein promote myotube growth through Akt/mTOR/S6K pathway. Finally, daidzein augments MyoD-dependent myogenic conversion of mouse embryonic fibroblasts. Taken together, our data suggest that daidzein promotes myogenic differentiation and myotube hypertrophy through p38 and Akt/mTOR/S6K pathway.