Does inflammation induced by ultraviolet B and heat rekindling alter pain-related behaviour in rats?. (September 2016)

• Record Type: Journal Article
• Authors: Smith, Melissa; Taylor, Collette; Weerasinghe, Nirosha; Koutsikou, Stella; Lumb, Bridget; Murrell, Jo
• Is Part Of: Veterinary anaesthesia and analgesia. Volume 43:Number 5(2016:Sep.)
• Page Start: 579
• Page End: 585
• Publication Date: 2016-09
• Subjects: burrowing -- heat rekindling -- pain -- rat -- ultraviolet B; Veterinary anesthesia -- Periodicals; 636.089
• DOI: 10.1111/vaa.12349
• Languages: English
• ISSNs: 1467-2987

Abstract

Abstract: Objective: To investigate whether induction of the ultraviolet B and heat rekindling (UVB/HR) model alters burrowing behaviour in rats. Study design: Randomized, blinded, prospective experimental study. Animals: Sixteen adult male Wistar rats weighing 250–300 g. Methods: In the UVB/HR group ( n = 8), UV irradiation was delivered to the heel area of the right plantar pelvic limb paw at a dose of 1000 mJ cm −2, using a narrow-band UVB light source. Twenty-four hours later, heat rekindling was performed by placement of a feedback-controlled thermode set at a constant temperature of 45 °C over the area of UVB irradiation for 5 minutes. Both interventions were carried out under pentobarbital anaesthesia. The 'sham' group ( n = 8) was anaesthetized only. In the burrowing test, rats were housed singly for 2 hours in cages furnished with a burrow filled with sand. The amount of sand remaining in the burrow after 2 hours was weighed and the amount displaced from the burrow calculated. The burrowing test was carried out for two consecutive days prior to UVB irradiation (day 0), on day 1 prior to HR, on days 2 and 3 after UVB exposure and at equivalent time points in the sham group. Results: Rats in the sham group burrowed means (SD) of 2429 (73) g and 2358 (124) g of sand on days −2 and 3, respectively, while those in the UVB/HR group burrowed 2460 (26) and 2419 (58) g on days −2 and 3, respectively. There was no significant effect of treatment on the amount of sand burrowed at any time point. Conclusions and clinical relevance: Pain associated with UVB/HR model induction is below the threshold required to affect rat burrowing behaviour and therefore questions the face validity of UVB/HR as a translational model of inflammatory pain.