Functional characterization of a reactive oxygen species modulator 1 gene in Litopenaeus vannamei. Issue 70 (November 2017)

• Record Type: Journal Article
• Authors: He, Hong-Hui; Chi, Yi-Miao; Yuan, Kai; Li, Xiao-Yun; Weng, Shao-Ping; He, Jian-Guo; Chen, Yi-Hong
• Is Part Of: Fish & shellfish immunology. Issue 70(2017)
• Page Start: 270
• Page End: 279
• Publication Date: 2017-11
• Subjects: Reactive oxygen species modulator 1 -- Reactive oxygen species -- Oxidative stress -- Litopenaeus vannamei -- White spot syndrome virus; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2017.09.024
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Reactive oxygen species (ROS) imparts a dual effect on multicellular organisms, wherein high levels are usually harmful, and low levels could facilitate in combating pathogenic microorganisms; therefore, the regulation of ROS production is critical. Previous studies have suggested that ROS contributes to resistance to the white spot syndrome virus (WSSV) or Vibrio alginolyticus in Litopenaeus vannamei . However, the regulation of ROS metabolism in L. vannamei remains elusive. In the present study, we proved that the overexpression of L. vannamei reactive oxygen species modulator 1 ( LvROMO1 ) increases ROS production in Drosophila Schneider 2 (S2) cells. Real-time RT-PCR analysis indicated that LvROMO1 is induced by WSSV or V. alginolyticus infection and β -glucan or microcystin (MC-LR) injection. Further investigation showed that LvROMO1 responding to MC-LR, thereby inducing hemocytes to undergo apoptosis, and ultimately resulting in hepatopancreatic damage. And LvROMO1 downregulation induced an increase in the cumulative mortality of WSSV-infected shrimp by reducing ROS production and suppressing the expression of antimicrobial peptides genes . The findings of present study suggest that LvROMO1 plays an important role in ROS production in L. vannamei and is involved in innate immunity. Highlights: Identification and characterization of a reactive oxygen species modulator 1 Gene in Litopenaeus vannamei ( LvROMO1 ). LvROMO1 contributes to MC-LR caused hemocytes apoptosis and hepatopancreas damage. Down-regulated expression of LvROMO1 increased the cumulative mortality of WSSV-infected shrimp via decreasing ROS level. Reducing expression of LvROMO1 increases the cumulative mortality of V. alginolyticus -infected shrimp.