Integration of Photosensors in a Nano-liter Scale Chromatography Column for the Online Monitoring of Adsorption/Desorption Kinetics of a Fluorophore-labeled Monoclonal Antibody. (2016)

• Record Type: Journal Article
• Authors: Pinto, Inês F.; Santos, D.R.; Soares, R.R.G.; Aires-Barros, M.R.; Chu, V.; Azevedo, A.M.; Conde, J.P.
• Is Part Of: Procedia engineering. Volume 168(2016)
• Page Start: 1426
• Page End: 1429
• Publication Date: 2016
• Subjects: On-chip chromatography -- Microfluidics -- a-Si:H photodiodes -- Fluorescence -- Monoclonal Antibodies -- Binding Kinetics; Engineering -- Congresses; Engineering -- Periodicals; Engineering; Conference proceedings; Periodicals; 620.005
• DOI: 10.1016/j.proeng.2016.11.405
• Languages: English
• ISSNs: 1877-7058

Abstract

Abstract: Chromatography is a robust purification technique, but requires time-consuming optimization on a case-by-case basis, particularly for monoclonal antibodies (mAbs). This work presents a novel microfluidic platform that significantly speeds up the optimization process and reduces the amount of reagent needed. Binding kinetics for a fluorescent conjugate mAb-Alexa 430 were measured in real time at resin level by integration of the microfluidic chip with 200×200 μm a-Si:H photodiodes on glass substrates aligned with micro-columns. Screening studies were performed using reduced amounts of reagents (∼50 μL), mAb molecules (∼2.5 μL) and resin (∼70 nL) in a rapid (∼2 min/condition) and simple manner.