Hypoxic X-irradiation as an external stimulus for conformational change of oligodeoxynucleotides that possess disulfide bond and regulation of DNAzyme function. Issue 2 (15th January 2015)

• Record Type: Journal Article
• Authors: Tanabe, Kazuhito; Okada, Kana; Sugiura, Masaaki; Ito, Takeo; Nishimoto, Sei-ichi
• Is Part Of: Bioorganic & medicinal chemistry letters. Volume 25:Issue 2(2015)
• Page Start: 310
• Page End: 312
• Publication Date: 2015-01-15
• Subjects: Hypoxic X-irradiation -- DNAzyme -- Disulfide bond -- Oligodeoxynucleotides; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1016/j.bmcl.2014.11.043
• Languages: English
• ISSNs: 0960-894X

Abstract

Graphical abstract: Abstract: We achieved a conformational change of oligodeoxynucleotides and the regulation of DNAzyme function by means of a radiolytic strand exchange reaction of disulfide bond. We designed a system in which the DNAzyme function of RNA cleavage was suppressed by the hybridization of an inhibitor strand that possessed disulfide bond with an active DNAzyme. Hypoxic X-irradiation led to the recovery of RNA cleavage because the strand exchange reaction at the disulfide bond in inhibitor strand resulted in a release of inhibitor strand. This strategy may be applicable to gene regulation by hypoxic X-irradiation.