Detection of Adrenaline Based on Substrate Recycling Amplification. (2015)

• Record Type: Journal Article
• Authors: Molinnus, D.; Bartz, A.; Bäcker, M.; Siegert, P.; Willenberg, H.; Poghossian, A.; Keusgen, M.; Schöning, M.J.
• Is Part Of: Procedia engineering. Volume 120(2015)
• Page Start: 540
• Page End: 543
• Publication Date: 2015
• Subjects: Adrenaline -- laccase -- biosensor -- substrate recycling ;; Engineering -- Congresses; Engineering -- Periodicals; Engineering; Conference proceedings; Periodicals; 620.005
• DOI: 10.1016/j.proeng.2015.08.708
• Languages: English
• ISSNs: 1877-7058

Abstract

Abstract: An amperometric enzyme biosensor has been applied for the detection of adrenaline. The adrenaline biosensor has been prepared by modification of an oxygen electrode with the enzyme laccase that operates at a broad pH range between pH 3.5 to pH 8. The enzyme molecules were immobilized via cross-linking with glutaraldehyde. The sensitivity of the developed adrenaline biosensor in different pH buffer solutions has been studied.