Chemical constituents of Miliusa balansae leaves and inhibition of nitric oxide production in lipopolysaccharide-induced RAW 264.7 cells. Issue 18 (15th September 2015)

• Record Type: Journal Article
• Authors: Thao, Nguyen Phuong; Luyen, Bui Thi Thuy; Tai, Bui Huu; Cuong, Nguyen Manh; Kim, Youn Chul; Minh, Chau Van; Kim, Young Ho
• Is Part Of: Bioorganic & medicinal chemistry letters. Volume 25:Issue 18(2015)
• Page Start: 3859
• Page End: 3863
• Publication Date: 2015-09-15
• Subjects: Miliusa balansae -- Annonaceae -- Milbasides A–C -- Anti-inflammatory; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1016/j.bmcl.2015.07.056
• Languages: English
• ISSNs: 0960-894X

Abstract

Graphical abstract: Abstract: Methanolic extract of Miliusa balansae Finet et Gagnep exerts an anti-inflammatory effect via inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophage cells. Three new megastigmane glycosides, milbasides A–C (1 –3 ), together with fifteen known compounds (4 –18 ), were isolated from the active fraction. Their chemical structures were elucidated using extensive spectroscopic analyses, including 1D and 2D NMR, HR ESI MS, and CD analysis, as well as comparison with previously reported data. Compounds1 –3, 11 and14 (20.0 μM) showed potent inhibitory activities with inhibition values of 98.5 ± 1.6%, 90.9 ± 7.8%, 84.8 ± 3.5%, 91.5 ± 8.7%, and 91.8 ± 2.7%, respectively. Our results suggest that megastigmane glycosides from M. balansae leaves may be used to treat inflammatory diseases.