Lactate Monitoring in Organotypic 3D Cell Cultures. (2015)

• Record Type: Journal Article
• Authors: Hammer, S.; Weltin, A.; Kaminski, Y.; Noor, F.; Kieninger, J.; Urban, G.A.
• Is Part Of: Procedia engineering. Volume 120(2015)
• Page Start: 961
• Page End: 964
• Publication Date: 2015
• Subjects: Cancer -- lactate -- microsensors -- electrochemical -- monitoring -- spheroids -- toxicology -- 3D cell culture; Engineering -- Congresses; Engineering -- Periodicals; Engineering; Conference proceedings; Periodicals; 620.005
• DOI: 10.1016/j.proeng.2015.08.825
• Languages: English
• ISSNs: 1877-7058

Abstract

Abstract: We present an electrochemical metabolic monitoring system, which is combined with a spheroid 3D tumor cell culture environment. It allows the continuous and precise monitoring of lactate production rates of single human hepatocyte spheroids online over days. Lactate concentration was measured using an electrochemical microsensor enabling the real-time monitoring of metabolic rates in combination with drug exposure. This setup can be used for drug testing as a powerful tool for fundamental research in general biology and toxicology.