Recombinant small glutamine-rich tetratricopeptide repeat-containing protein of Leishmania infantum: Potential vaccine and diagnostic application against visceral leishmaniasis. (November 2017)

• Record Type: Journal Article
• Authors: Dias, Daniel S.; Ribeiro, Patrícia A.F.; Martins, Vívian T.; Lage, Daniela P.; Portela, Áquila S.B.; Costa, Lourena E.; Salles, Beatriz C.S.; Lima, Mariana P.; Ramos, Fernanda F.; Santos, Thaís T.O.; Caligiorne, Rachel B.; Chávez-Fumagalli, Miguel A.; Silveira, Julia A.G.; Magalhães-Soares, Danielle F.; Gonçalves, Denise U.; Oliveira, Jamil S.; Roatt, Bruno M.; Duarte, Mariana C.; Menezes-Souza, Daniel; Silva, Eduardo S.; Galdino, Alexsandro S.; Machado-de-Ávila, Ricardo A.; Teixeira, Antonio L.; Coelho, Eduardo A.F.
• Is Part Of: Molecular immunology. Volume 91(2017:Nov.)
• Page Start: 272
• Page End: 281
• Publication Date: 2017-11
• Subjects: Visceral leishmaniasis -- Small glutamine-rich tetratricopeptide repeat-containing protein -- Vaccine -- Serological diagnosis; Immunochemistry -- Periodicals; Molecular biology -- Periodicals; Immunochemistry -- Periodicals; Allergy and Immunology -- Periodicals; Molecular Biology -- Periodicals; Immunochimie -- Périodiques; Biologie moléculaire -- Périodiques; Immunochemistry; Molecular biology; Periodicals; Electronic journals; 571.96
• DOI: 10.1016/j.molimm.2017.09.017
• Languages: English
• ISSNs: 0161-5890

Abstract

Graphical abstract: Highlights: Use of the Leishmania SGT protein against visceral leishmaniasis. Serological marker to identify VL patients, but without presents cross-reactivity. Partial protection induced in BALB/c mice against L. infantum infection. Immunogenicity in PBMCs from recovered and treated VL patients with IFN-γ production. A new candidate to studies as vaccine or serological marker against human VL. Abstract: Different Leishmania proteins have been evaluated in order to find a potential vaccine candidate or diagnostic marker capable of providing long lasting protection against infection or helping to identify infected mammalian hosts, respectively. However, just few molecules have fulfilled all the requirements to be evaluated. In the current study, we evaluated the prophylactic and diagnostic value against visceral leishmaniasis (VL) of a small glutamine-rich tetratricopeptide repeat-containing (SGT) protein from Leishmania infantum species. In a first step, the immune response elicited by the immunization using the recombinant protein (rSGT) plus saponin was evaluated in BALB/c mice. Immunized animals had a low parasitism in all evaluated organs. They developed a specific Th1 immune response, which was based on protein-specific production of IFN-γ, IL-12 and GM-CSF, and a humoral response dominated by antibodies of the IgG2a isotype. Both CD4 + and CD8 + T cells contributed to the IFN-γ production, showing that both T cell subtypes contribute to the resistance against infection. Regarding its value as a diagnostic marker, rSGT showed maximum sensitivity and specificity to serologically identify L. infantum -infected dog and human sera. No cross-reactivity with sera from humans or dogs that had other diseases was found. Although further studies are necessary to validate these findings, data showed here suggest immunogenicity of rSGT and its protective effect against murine VL, as well as its potential for the serodiagnosis of human and canine VL.