Ecotoxicity of bisphenol A to Caenorhabditis elegans by multigenerational exposure and variations of stress response in vivo across generations. (January 2016)

• Record Type: Journal Article
• Authors: Zhou, Dong; Yang, Jie; Li, Hui; Lu, Qiang; Liu, Yong-di; Lin, Kuang-fei
• Is Part Of: Environmental pollution. Volume 208:Part B(2016)
• Page Start: 767
• Page End: 773
• Publication Date: 2016-01
• Subjects: Caenorhabditis elegans -- Bisphenol A -- Multigenerational exposure -- Multigenerational toxicity response -- Environmental risks; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2015.10.057
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: In order to understand how bisphenol A (BPA) exposure acts on the evolutionary dynamics of populations and changes of stress response across generations, the model animal Caenorhabditis elegans was used to conduct the multigenerational testing. Multiple endpoints at the physiological (growth, reproduction, and locomotion behaviors) and molecular (stress-related gene expressions) levels were examined by multigenerational exposure to low-concentration BPA (0.001–10 μM) across four generations. The results showed that changes of physiological-level effects across four generations varied in magnitude and direction, depending on the exposure concentrations. C. elegans individuals in the first generation grew smaller, moved slower, and produced less offsprings than the controls by BPA exposure. As for each trait tested, the first generation response could be commonly mirrored in the subsequent generations at the highest concentration of 10 μM. However, at lower concentrations, response of parental generation was a relatively poor predictor of the effects on progeny, as acclimation or cumulative damage could occur in the subsequent generations. The integrated gene expression profiles visually illustrated that the tested gene expressions at low concentrations (0.001–0.01 μM) were more obviously changed in both G1 and G4 generations, and the G1 generation showed a much greater degree of increase in stress-related gene expressions than the G4 generation. The multigenerational toxicity data emphasize the need of considering biological effects over multiple generations to conduct accurate assessment of environmental risks of toxicants on population dynamics. Graphical abstract: Highlights: Multigenerational exposure was conducted to evaluate effects of BPA on C. elegans. Changes of biological effects varied in magnitude and direction across generations. A more significant induction in stress-related gene expressions was found in G1 . Multigenerational tests have great relevance for environmental risk assessment. Abstract : Multigenerational exposure was conducted on Caenorhabditis elegans to better understand biological impacts of BPA across generations and changes of stress response in vivo .