Impact of using validated or standard reference genes for miRNA qPCR data normalization in cell type identification. (December 2015)

• Record Type: Journal Article
• Authors: Sirker, M.; Liang, W.; Zhang, L.; Fimmers, R.; Rothschild, M.A.; Gomes, I.; Schneider, P.M.
• Is Part Of: Forensic science international. Volume 5(2015)
• Page Start: e199
• Page End: e201
• Publication Date: 2015-12
• Subjects: Forensic science -- miRNA profiling -- Body fluid identification -- Reference genes -- GeNorm -- Normalization; Forensic genetics -- Periodicals; Forensic Genetics -- Periodicals; Electronic journals; 614.1
• DOI: 10.1016/j.fsigss.2015.09.080
• Languages: English
• ISSNs: 1875-1768

Abstract

Abstract: For the analysis of cell type-specific miRNA expression patterns qPCR is currently the method of choice owing to its high accuracy. However, to obtain reliable results, a proper normalization strategy is an absolute prerequisite, which is often underestimated. To demonstrate the importance of using a set of suitable reference genes, we tested two normalization strategies by comparing gene expressions of tissue-specific miRNA targets normalized against: (1) previously validated endogenous controls (miR92 and miR374) and (2) a commonly used miRNA reference (U6B).