Electrostatic Detection of Unlabelled Single- and Double-stranded DNA Using Capacitive Field-effect Devices Functionalized with a Positively Charged Polyelectrolyte Layer. (2015)

• Record Type: Journal Article
• Authors: Bronder, T.S.; Poghossian, A.; Scheja, S.; Wu, C.S.; Keusgen, M.; Schöning, M.J.
• Is Part Of: Procedia engineering. Volume 120(2015)
• Page Start: 544
• Page End: 547
• Publication Date: 2015
• Subjects: label-free detection -- DNA biosensor -- field-effect sensor -- polyelectrolyte; Engineering -- Congresses; Engineering -- Periodicals; Engineering; Conference proceedings; Periodicals; 620.005
• DOI: 10.1016/j.proeng.2015.08.710
• Languages: English
• ISSNs: 1877-7058

Abstract

Abstract: Capacitive field-effect electrolyte-insulator-semiconductor sensors consisting of an Al-p-Si-SiO2 structure have been used for the electrical detection of unlabelled single- and double-stranded DNA (dsDNA) molecules by their intrinsic charge. A simple functionalization protocol based on the layer-by-layer (LbL) technique was used to prepare a weak polyelectrolyte/probe-DNA bilayer, followed by the hybridization with complementary target DNA molecules. Due to the flat orientation of the LbL-adsorbed DNA molecules, a high sensor signal has been achieved. In addition, direct label-free detection of in-solution hybridized dsDNA molecules has been studied.