Grape seed extract supplementation attenuates the heat stress-induced responses of jejunum epithelial cells in Simmental × Qinchuan steers. Issue 3 (20th May 2014)

• Record Type: Journal Article
• Authors: Li, Xiaomin; Yang, You; Liu, Shimin; Yang, Jing; Chen, Cheng; Sun, Zhihong
• Is Part Of: British journal of nutrition. Volume 112:Issue 3(2014)
• Page Start: 347
• Page End: 357
• Publication Date: 2014-05-20
• Subjects: Immune responses, -- Grape seed extract, -- Jejunum epithelial cells, -- Heat stress, -- Steers; Nutrition -- Periodicals; 572.4
• DOI: 10.1017/S0007114514001032
• Languages: English
• ISSNs: 0007-1145

Abstract

Abstract : Grape seed extract (GSE), a rich source of polyphenols, is reported to possess antioxidant, anti-inflammatory and immunomodulatory properties. The objective of the present study was to determine whether GSE could attenuate the heat stress-induced responses of jejunum epithelial cells (JEC) in cattle. The JEC of a steer (Simmental × Qinchuan) were exposed to heat stress for 2 h in the absence (0 μg/ml) or presence (10, 20, 40 and 80 μg/ml) of GSE in the culture medium. When cultured at 40°C, JEC supplemented with GSE exhibited increased glutathione peroxidase activity ( P = 0·04), viability ( P = 0·004), and mRNA expression of epidermal growth factor ( EGF ; P = 0·03) and EGF receptor ( EGFR ; P = 0·01). Under the same conditions, the cells exhibited decreased mRNA expression of IL-8 ( P = 0·01) and TNF-α ( P = 0·03) and decreased protein concentrations of IL-1β ( P = 0·02), Toll-like receptor 4 (TLR4; P = 0·04) and heat shock protein 70 (HSP70; P < 0·001). When cultured at 43°C, JEC supplemented with GSE exhibited increased catalase activity ( P = 0·04), viability ( P < 0·001), and mRNA expression of EGF ( P < 0·001) and EGFR ( P < 0·001) and decreased protein concentrations of IL-1β ( P < 0·001), TLR4 ( P = 0·03) and HSP70 ( P < 0·001), as well as mRNA expression of IL-8 ( P < 0·001), TLR4 ( P = 0·002) and TNF-α ( P < 0·001). Temperature × GSE concentration interactions were also observed for the concentrations of IL-1β ( P < 0·001), IL-8 ( P < 0·001), TNF-α ( P = 0·01) and HSP70 ( P = 0·04) and viability ( P < 0·001) of JEC. The results of the present study indicate that GSE can attenuate the responses of JEC induced by heat stress within a certain range of temperatures.