Liver volatolomics to reveal poultry exposure to γ-hexabromocyclododecane (HBCD). (December 2017)

• Record Type: Journal Article
• Authors: Ratel, Jérémy; Planche, Christelle; Mercier, Frédéric; Blinet, Patrick; Kondjoyan, Nathalie; Marchand, Philippe; Fournier, Agnès; Travel, Angélique; Jondreville, Catherine; Engel, Erwan
• Is Part Of: Chemosphere. Volume 189(2017)
• Page Start: 634
• Page End: 642
• Publication Date: 2017-12
• Subjects: Food safety -- γ-HBCD -- Animal-derived food -- Volatolomics -- Liver -- SPME-GC-MS; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2017.09.074
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Hexabromocyclododecane (HBCD) is a critical emerging brominated flame retardant to which consumers can be exposed at high doses through a single food intake. Based on an animal experiment involving 3 groups of laying hens fed during 70 days with a control diet or γ-HBCD-contaminated diets at 0.1 or 10 μg γ-HBCD g −1 feed, this study aims to use the volatolome of biological samples for revealing markers of livestock exposure to HBCD. Liquid chromatography–tandem mass spectrometry was used to monitor the time-course of HBCD levels in bodily samples. Each liver was analyzed by solid-phase microextraction–gas chromatography–mass spectrometry for volatolome profiling. After 70 days, γ-HBCD concentrations in egg yolk, fat, liver and serum reached 54 ± 4, 85 ± 6, 31 ± 6, and 32 ± 4 ng g −1 lw, respectively, for the low exposure level and 4.6+/5.7, 7.8+/6.5, 3.9+/3.0 and 3.9+/6.1 μg g −1 lw, respectively, for the high exposure level. Isomerization of γ-HBCD into α- and β-HBCD was observed in all tissues, at least for the high exposure level. Volatolome data allowed a significant discrimination between control and exposed animals whatever the feed contamination load, demonstrating a liver metabolic response to γ-HBCD exposure. The relevance of the twenty nine volatile exposure markers tentatively identified was discussed in light of literature data. Graphical abstract: Highlights: Poultry liver volatolome is modified by the dietary exposure of animals to γ-HBCD. Volatile candidate markers in liver enable to reveal livestock exposure to γ-HBCD. Candidate markers are mainly hydrocarbons or oxygenated compounds. Liver volatolomics is a promising approach to strengthen food contamination control.