DNA barcoding of five common stored-product pest species of genus Cryptolestes (Coleoptera: Laemophloeidae). Issue 4 (11th April 2014)

• Record Type: Journal Article
• Authors: Wang, Y.J.; Li, Z.H.; Zhang, S.F.; Varadínová, Z.; Jiang, F.; Kučerová, Z.; Stejskal, V.; Opit, G.; Cao, Y.; Li, F.J.
• Is Part Of: Bulletin of entomological research. Volume 104:Issue 4(2014)
• Page Start: 486
• Page End: 493
• Publication Date: 2014-04-11
• Subjects: Cryptolestes, -- stored-product pests, -- identification, -- DNA barcoding, -- COI; Entomology -- Periodicals; 595.7
• DOI: 10.1017/S0007485314000224
• Languages: English
• ISSNs: 0007-4853

Abstract

Abstract: Several species of the genus Cryptolestes Ganglbauer, 1899 (Coleoptera: Laemophloeidae) are commonly found in stored products. In this study, five species of Cryptolestes, with almost worldwide distribution, were obtained from laboratories in China, Czech Republic and the USA: Cryptolestes ferrugineus (Stephens, 1831), Cryptolestes pusillus (Schönherr, 1817), Cryptolestes turcicus (Grouvelle, 1876), Cryptolestes pusilloides (Steel & Howe, 1952) and Cryptolestes capensis (Waltl, 1834). Molecular identification based on a 658 bp fragment from the mitochondrial DNA cytochrome c oxidase subunit I (COI) was adopted to overcome some problems of morphological identification of Cryptolestes species. The utility of COI sequences as DNA barcodes in discriminating the five Cryptoleste s species was evaluated on adults and larvae by analysing Kimura 2-parameter distances, phylogenetic tree and haplotype networks. The results showed that molecular approaches based on DNA barcodes were able to accurately identify these species. This is the first study using DNA barcoding to identify Cryptolestes species and the gathered DNA sequences will complement the biological barcode database.