Factors affecting storage of Slovak native rabbit semen in the gene bank. Issue 5 (24th August 2017)
- Record Type:
- Journal Article
- Title:
- Factors affecting storage of Slovak native rabbit semen in the gene bank. Issue 5 (24th August 2017)
- Main Title:
- Factors affecting storage of Slovak native rabbit semen in the gene bank
- Authors:
- Kulíková, Barbora
Oravcová, Marta
Baláži, Andrej
Supuka, Peter
Chrenek, Peter - Abstract:
- Summary: In this study, fresh and frozen–thawed semen of Nitra and Zobor rabbit breeds were evaluated for potential inter-breed or inter-male differences in sperm quality traits. Individual male semen from four rabbits of each breed were diluted (v:v; 1:1) in a freezing medium composed of a commercial diluent, 16% of dimethyl sulphoxide (DMSO), 4% of Ficoll 70 and 2% of sucrose and frozen in liquid nitrogen vapours before being plunged into liquid nitrogen. Different motility traits, viability and plasma membrane integrity of fresh and frozen–thawed semen were evaluated in vitro using computer-assisted sperm analysis and flow cytometry. To evaluate the sperm fertilization ability, artificial insemination of fresh and frozen–thawed sperm was performed. Our results showed the effect of breed ( P ≤ 0.05) on frozen–thawed sperm viability and plasma membrane integrity. Moreover, individual variability in semen quality among the rabbits was revealed (0.31 to 0.71 among quality traits). Our results thereby confirmed that the cryopreservation procedure could not ensure comparable sperm post-thaw survival for different breeds or males. Nevertheless, correlations between numbers of fresh total motile and progressively moving sperm and several quality parameters measured post thawing were revealed. Therefore, we suggest that the objective assessment of fresh rabbit sperm motility may be an effective indicator of frozen–thawed semen quality. Consequently, regular semen assessment isSummary: In this study, fresh and frozen–thawed semen of Nitra and Zobor rabbit breeds were evaluated for potential inter-breed or inter-male differences in sperm quality traits. Individual male semen from four rabbits of each breed were diluted (v:v; 1:1) in a freezing medium composed of a commercial diluent, 16% of dimethyl sulphoxide (DMSO), 4% of Ficoll 70 and 2% of sucrose and frozen in liquid nitrogen vapours before being plunged into liquid nitrogen. Different motility traits, viability and plasma membrane integrity of fresh and frozen–thawed semen were evaluated in vitro using computer-assisted sperm analysis and flow cytometry. To evaluate the sperm fertilization ability, artificial insemination of fresh and frozen–thawed sperm was performed. Our results showed the effect of breed ( P ≤ 0.05) on frozen–thawed sperm viability and plasma membrane integrity. Moreover, individual variability in semen quality among the rabbits was revealed (0.31 to 0.71 among quality traits). Our results thereby confirmed that the cryopreservation procedure could not ensure comparable sperm post-thaw survival for different breeds or males. Nevertheless, correlations between numbers of fresh total motile and progressively moving sperm and several quality parameters measured post thawing were revealed. Therefore, we suggest that the objective assessment of fresh rabbit sperm motility may be an effective indicator of frozen–thawed semen quality. Consequently, regular semen assessment is required in order to preserve good-quality insemination doses from native breeds. … (more)
- Is Part Of:
- Zygote. Volume 25:Issue 5(2017)
- Journal:
- Zygote
- Issue:
- Volume 25:Issue 5(2017)
- Issue Display:
- Volume 25, Issue 5 (2017)
- Year:
- 2017
- Volume:
- 25
- Issue:
- 5
- Issue Sort Value:
- 2017-0025-0005-0000
- Page Start:
- 592
- Page End:
- 600
- Publication Date:
- 2017-08-24
- Subjects:
- Cryopreservation, -- Quality trait, -- Rabbit, -- Sperm, -- Variability
Developmental biology -- Periodicals
Embryology -- Periodicals
Zygotes -- Periodicals
Gametes -- Periodicals
571.864 - DOI:
- 10.1017/S0967199417000454 ↗
- Languages:
- English
- ISSNs:
- 0967-1994
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library HMNTS - ELD Digital store
- Ingest File:
- 4787.xml