Comparative Analysis of Essential Oil and Different Extracts of Ajuga chamaecistus ssp. scoparia on Antibacterial Activity. Issue 4 (4th July 2017)

• Record Type: Journal Article
• Authors: Mohammadi-Bazargani, Mitra; Rohloff, Jens; Atapour, Maryam
• Is Part Of: Journal of essential oil-bearing plants. Volume 20:Issue 4(2017)
• Page Start: 1117
• Page End: 1124
• Publication Date: 2017-07-04
• Subjects: Ajuga chamaecistus ssp. scoparia -- antibacterial activity -- Escherichia coli -- essential oil (EO) -- plant extract -- Staphylococcus aureus; Essences and essential oils -- Periodicals; Aromatic plants -- Biotechnology -- Periodicals; Oilseed plants -- Biotechnology -- Periodicals; Aromatic plants -- Periodicals; Oilseed plants -- Periodicals; 661.80605
• DOI: 10.1080/0972060X.2017.1365017
• Languages: English
• ISSNs: 0972-060X

Abstract

Abstract: Antibacterial activities of essential oils (EO) and different plant extracts obtained from the aerial parts of Ajuga chamaecistus ssp. scopria were investigated under in vitro condition. Minimum inhibitory concentration (MIC) assay was carried out using two-fold serial dilution method and MTT assay against gram-positive ( Staphylococcus aureus ) and gram-negative ( Escherichia coli ) bacteria. Antibacterial activity against both bacteria was observed for the EO, while the hexane extract exhibited antibacterial effect only against S. aureus . The EO analysis with GC and GC-MS exhibited sixty nine compounds representing 99.72% of the total oil composition. The major constituents were β-pinene (16.08%), α-thujene (10.66%), α-pinene (7.43%), linalool (7.37%), bicyclogermacrene (6.71%), δ-cadinene (6.40%), limonene (4.95%) and spathulenol (3.09%). The antibacterial activity of EO observed in the present study could be due to the major compounds belonging to oxygenated terpenes and aromatic structures, the large monoterpenes group and potential synergistic effects between these compounds and other terpenes.