Crystallization via tubing microfluidics permits both in situ and ex situ X‐ray diffraction. Issue 10 (10th October 2017)

• Record Type: Journal Article
• Authors: Gerard, Charline J. J.; Ferry, Gilles; Vuillard, Laurent M.; Boutin, Jean A.; Chavas, Leonard M. G.; Huet, Tiphaine; Ferte, Nathalie; Grossier, Romain; Candoni, Nadine; Veesler, Stéphane
• Is Part Of: Acta crystallographica. Volume 73:Issue 10(2017:Oct.)
• Page Start: 574
• Page End: 578
• Publication Date: 2017-10-10
• Subjects: crystallization -- microfluidics -- optimization -- in situ X‐ray diffraction -- ex situ X‐ray diffraction; Crystallography -- Periodicals; Crystals -- Periodicals; 548
• DOI: 10.1107/S2053230X17013826
• Languages: English
• ISSNs: 2053-230X

Abstract

Abstract : Crystallization conditions for a pharmaceutical protein were optimized using a microfluidic platform and X‐ray data were collected both in situ and ex situ . Abstract : A microfluidic platform was used to address the problems of obtaining diffraction‐quality crystals and crystal handling during transfer to the X‐ray diffractometer. Crystallization conditions of a protein of pharmaceutical interest were optimized and X‐ray data were collected both in situ and ex situ .