In-vitro behavior of different fully dense calcium phosphate materials fabricated by Spark Plasma Sintering. Issue 18 (15th December 2017)

• Record Type: Journal Article
• Authors: Mancuso, Luisa; Desogus, Luca; Orrù, Roberto; Loy, Francesco; Cao, Giacomo
• Is Part Of: Ceramics international. Volume 43:Issue 18(2017)
• Page Start: 16238
• Page End: 16247
• Publication Date: 2017-12-15
• Subjects: In-vitro test -- Osteoblasts -- Mineralization -- Calcium phosphates -- Spark Plasma Sintering -- Microstructure; Ceramics -- Periodicals; Céramique industrielle -- Périodiques; Ceramics; Periodicals; Electronic journals; 666
• DOI: 10.1016/j.ceramint.2017.08.208
• Languages: English
• ISSNs: 0272-8842

Abstract

Abstract: Results obtained during in-vitro experiments concerning human osteoblasts cultivated on the surface of dense samples produced by Spark Plasma Sintering from three types of hydroxyapatite powders are described and discussed. The sintered products display diverse composition and microstructures which are found to significantly influence the biological response of the cells. Osteoblasts adhesion, viability and proliferation are quantitatively comparable for the three classes of bioceramics, whereas matrix mineralization occurs only in products exclusively consisting of hydroxyapatite. Correspondingly, a calcium-phosphate layer exhibiting a trabecular-like microstructure is deposited on the materials surface. Matrix mineralization is favored when the substrate is composed of submicrometer-sized apatite grains. On the other hand, the latter phenomena is markedly suppressed, and so does the formation of the new apatite phase, when cells are seeded on sintered disks composed of β-Tri-Calcium Phosphate, which was formed during the sintering process from the decomposition of initial apatite.