Doxorubicin Adsorbed on Carbon Nanotubes: Helical Structure and New Release Trigger. Issue 19 (25th August 2017)

• Record Type: Journal Article
• Authors: Sadaf, Shamaila; Walder, Lorenz
• Is Part Of: Advanced materials interfaces. Volume 4:Issue 19(2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-08-25
• Subjects: carbon nanotubes -- doxorubicin -- electrochemistry -- eQCM -- glutathione -- semi‐empirical -- STM; Materials science -- Periodicals; 620.11
• DOI: 10.1002/admi.201700649
• Languages: English
• ISSNs: 2196-7350

Abstract

Abstract: The well‐known drug delivery system "doxorubicin physically loaded on carbon nanotubes" (Dox@CNT) is visualized by scanning tunneling microscopy at the molecular level, revealing rich architectural variability of Dox@CNT, and allowing to measure and rationalize reported loading efficiencies (80–200%) for the first time from image analysis. Reduction of Dox@CNT is identified as a so far unknown intrinsic release mechanism of biochemically relevance for Dox from Dox@CNT requiring no further CNT surface modification beside Dox loading. Electron injection into Dox@CNT from an electrode or from the biological reducing agent glutathione (GSH) leads to irreversible release of Dox. Its rate follows a linear free energy relationship (reduction potential vs log (Dox release rate)) with half‐life times from below seconds to hours. With extracellular GSH levels in the micromolar range and intracellular GSH concentrations of 10 × 10 −3 m or even higher, the findings can explain the preferential intracellular release of Dox from its physically adsorbed state on CNTs. The influence of acidity on the release rate of Dox on pristine 6, 5‐CNTs in the absence of GSH is found to be negligible. The experimental findings are strongly supported by semi‐empirical calculations. Abstract : Doxorubicin self‐assembles on 6, 5 carbon nanotubes . Structural variability of the drug on its carrier is observed by scanning tunneling microscopy allowing to calculate loading efficiencies from image analysis. A new release mechanisme is presented. Electron injection from glutathione (GSH) or from an electrode into the Doxorubicin/CNT complex expells the drug. The mechanism is supported by theoretical modeling.