Transient transfection of Cryptosporidium baileyi. Issue 6 (December 2017)

• Record Type: Journal Article
• Authors: Tetens, A.-K.; Hanig, S.; Kurth, M.; Greif, G.; Entzeroth, R.
• Is Part Of: Parasitology international. Volume 66:Issue 6(2017:Dec.)
• Page Start: 813
• Page End: 816
• Publication Date: 2017-12
• Subjects: Transfection -- Apicomplexa -- Cryptosporidium -- Heterologous -- In ovo; Parasitology -- Periodicals; Parasites -- Periodicals; Parasitic Diseases -- Periodicals; Parasitology -- Periodicals; Parasitologie -- Périodiques; 571.99905
• DOI: 10.1016/j.parint.2017.09.005
• Languages: English
• ISSNs: 1383-5769

Abstract

Abstract: Here we demonstrate the transient transfection of C. baileyi . We describe the transfection of this apicomplexan parasite and the cultivation in ovo . The functionality of heterologous sequences in C. baileyi was demonstrated by the expression and detection of the mCherry protein in ovo . The mCherry protein was expressed in parasitic stages up to the oocyst stage under the control of the heterologous promoter region of the C. parvum actin gene.