Interleukin-13/interleukin-4 receptor pathway is crucial for production of Sda-sialomucin in mouse small intestinal mucosa by Nippostrongylus brasiliensis infection. Issue 6 (December 2017)

• Record Type: Journal Article
• Authors: Tsubokawa, Daigo; Ishiwata, Kenji; Goso, Yukinobu; Nakamura, Takeshi; Hatta, Takeshi; Ishihara, Kazuhiko; Kanuka, Hirotaka; Tsuji, Naotoshi
• Is Part Of: Parasitology international. Volume 66:Issue 6(2017:Dec.)
• Page Start: 731
• Page End: 734
• Publication Date: 2017-12
• Subjects: Mucin -- Il-13 -- Il-4R -- Nematode -- Nippostrongylus brasiliensis -- Sda antigen; Parasitology -- Periodicals; Parasites -- Periodicals; Parasitic Diseases -- Periodicals; Parasitology -- Periodicals; Parasitologie -- Périodiques; 571.99905
• DOI: 10.1016/j.parint.2017.08.004
• Languages: English
• ISSNs: 1383-5769

Abstract

Abstract: Mucin is a major component of mucus in gastrointestinal mucosa. Increase of specific sialomucins having Sd a blood group antigen, NeuAcα2-3(GalNAcβ1-4)Galβ1-4GlcNAcβ-, is considered to be associated with expulsion of the parasitic intestinal nematode Nippostrongylus brasiliensis . In this study, we examined the relationship between interleukin (IL)-13 pathway and expression of Sd a -sialomucins in small intestinal mucosa with N. brasiliensis infection. Nematode infection induced marked increases in small intestinal mucins that reacted with anti-Sd a antibody in wild type (wt) mice. However, this increase due to infection was supressed in IL-4 receptor α deficient (IL-4Rα −/− ) mice, which lack both IL-4 and IL-13 signaling via IL-4R, and severe combined immunodeficient (SCID) mice, which have defects in B- and T-lymphocytes. Analysis using tandem mass spectroscopy showed that Sd a -glycans were not expressed in small intestinal mucins in IL-4Rα −/− and SCID mice after infection despite the appearance of Sd a -glycans in the infected wt mice. Inoculation of recombinant IL-13 into the infected SCID mice restored expression of Sd a -glycan. Our results suggest that the IL-13/IL-4R axis is important for the production of Sd a -sialomucins in the host intestinal mucosa with parasitic nematode infection. Graphical abstract: Highlights: Sd a -sialomucins increase in mouse intestinal mucosa after N. brasiliensis infection. The increase in Sd a -sialomucins was suppressed in IL-4Rα −/− and SCID mice. The expression of Sd a -glycans was abrogated in IL-4Rα −/− and SCID mice. Administration of IL-13 led to expression of Sd a -glycan in infected SCID mice. IL-13/IL-4R axis is important for Sd a -sialomucin production during infection.