Ischemic stroke damages the intestinal mucosa and induces alteration of the intestinal lymphocytes and CCL19 mRNA in rats. (29th September 2017)

• Record Type: Journal Article
• Authors: Liu, Yaning; Luo, Shijian; Kou, Li; Tang, Chaogang; Huang, Ruxun; Pei, Zhong; Li, Zhendong
• Is Part Of: Neuroscience letters. Volume 658(2017)
• Page Start: 165
• Page End: 170
• Publication Date: 2017-09-29
• Subjects: GALT gut associated lymphoid tissue -- PPs Peyer's patches -- IELs intraepithelial lymphocytes -- MCAO middle cerebral artery occlusion -- CCA common carotid artery -- ICA internal carotid artery -- ECA external carotid artery -- FBS fetal bovine serum; Ischemic stroke -- Intestine -- Lymphocyte -- CCL25 -- CCL19; Neurology -- Periodicals; Neurology -- Periodicals; Research -- Periodicals; Neurologie -- Périodiques; Neuroanatomie -- Périodiques; Neuropharmacologie -- Périodiques; Neurophysiologie -- Périodiques; Neurology; Periodicals; Electronic journals; 617.48
• DOI: 10.1016/j.neulet.2017.08.061
• Languages: English
• ISSNs: 0304-3940

Abstract

Highlights: Studies have shown that peripheral immune activity is involved in brain injury after stroke. However, immune alteration and possible mechanisms in the intestine after stroke remain unclear. In ischemic stroke rats, we found significant epithelial injury, increased T lymphocytes in Peyer's patches, and transient upregulation of CCL19 mRNA expression in the intestine. The results suggested that ischemic stroke significantly damaged the intestinal mucosa and activated intestinal immunity, which might participate in the pathophysiology of stroke. Abstract: The immunoreaction has a pivotal effect on ischemic stroke. It has been demonstrated that intestinal lymphocytes infiltrate into the brain and aggravate tissue injury after stroke. However, less attention has been paid to the influence on the intestinal immunology as well as morphology. Here, we utilized a rat permanent middle cerebral artery occlusion (MCAO) model to investigate the influences on intestinal mucosa, lymphocytes of the gut-associated lymphoid tissue (GALT), and the intestinal expression of CCL25 mRNA and CCL19 mRNA of stroke. Rats were randomly divided into stroke, sham, and control groups. Stroke and sham groups were further divided into interval groups of 6 h, 12 h, and 24 h after surgery. Intestinal pathophysiological changes were observed by hematoxylin-eosin (H&E) staining. The lymphocyte numbers were detected by flow cytometry. The expression of CCL25 mRNA and CCL19 mRNA was tested with the PCR technique. We found significant necrosis and shedding of the epithelium after stroke. Moreover, the lesion aggravated with time. In addition, there was a significant increase of T lymphocytes in Peyer's patches (PPs), especially at 12 h and 24 h after stroke, while no differences in the number of B lymphocytes and the intraepithelial lymphocytes (IELs) were found. The data displayed no alteration of CCL25 mRNA expression. In contrast, an upregulation of CCL19 mRNA expression was detected at 6 h after stroke. This study showed that ischemic stroke significantly damaged the intestinal epithelium and activated intestinal immunity.