Identification and characterization of a rabbit novel IFN-α unlocated in genome. (January 2018)
- Record Type:
- Journal Article
- Title:
- Identification and characterization of a rabbit novel IFN-α unlocated in genome. (January 2018)
- Main Title:
- Identification and characterization of a rabbit novel IFN-α unlocated in genome
- Authors:
- Gao, Mingchun
Liu, Ying
Guo, Yongli
Wang, Yujiao
Dai, Haiyue
Song, Zhifeng
Wang, Junwei
Han, Wenyu - Abstract:
- Abstract: The multigene family of rabbit IFN-α (RbIFN-α) is located on chromosome 1, which shows seven functional genes in type I IFN locus. A novel RbIFN-α that remains unlocated in the rabbit genome was amplified and designated as the first novel rabbit IFN-α (RbIFN-αNov1), which possesses the typical molecular characteristics of type I IFNs and could be induced in RK-13 cells and peripheral blood mononuclear cells. After the mature peptide of RbIFN-αNov1 was expressed, its antiviral activity, physicochemical characteristics, and cytotoxicity were determined in vitro. Results indicated that RbIFN-αNov1 exerted a high specific antiviral activity against VSV and a low cytotoxic effect on RK-13 cells. RbIFN-αNov1 showed high sensitivity to trypsin and remained relatively stable after acid, alkali, or heat treatment. RbIFN-αNov1 could induce Mx1 expression on RK-13 cells and activate the NF-κB, ISRE and BoIFN-β promoter activities on bovine testicular cells. Overall, our research on RbIFN-αNov1 not only enriches the knowledge about rabbit IFNs but also makes RbIFN-αNov1 have the potential to be used as an effective therapeutic agent for rabbit viral diseases. Highlights: Rabbit type I interferon locus was constructed and analyzed. An unlocated rabbit IFN-α designated as RbIFN-αNov1 was characterized. RbIFN-αNov1 has broad antiviral activity and antiproliferative activity. RbIFN-αNov1 can induce Mx1 expression to exert antiviral activity. RbIFN-αNov1 can induce NF-κB, ISRE andAbstract: The multigene family of rabbit IFN-α (RbIFN-α) is located on chromosome 1, which shows seven functional genes in type I IFN locus. A novel RbIFN-α that remains unlocated in the rabbit genome was amplified and designated as the first novel rabbit IFN-α (RbIFN-αNov1), which possesses the typical molecular characteristics of type I IFNs and could be induced in RK-13 cells and peripheral blood mononuclear cells. After the mature peptide of RbIFN-αNov1 was expressed, its antiviral activity, physicochemical characteristics, and cytotoxicity were determined in vitro. Results indicated that RbIFN-αNov1 exerted a high specific antiviral activity against VSV and a low cytotoxic effect on RK-13 cells. RbIFN-αNov1 showed high sensitivity to trypsin and remained relatively stable after acid, alkali, or heat treatment. RbIFN-αNov1 could induce Mx1 expression on RK-13 cells and activate the NF-κB, ISRE and BoIFN-β promoter activities on bovine testicular cells. Overall, our research on RbIFN-αNov1 not only enriches the knowledge about rabbit IFNs but also makes RbIFN-αNov1 have the potential to be used as an effective therapeutic agent for rabbit viral diseases. Highlights: Rabbit type I interferon locus was constructed and analyzed. An unlocated rabbit IFN-α designated as RbIFN-αNov1 was characterized. RbIFN-αNov1 has broad antiviral activity and antiproliferative activity. RbIFN-αNov1 can induce Mx1 expression to exert antiviral activity. RbIFN-αNov1 can induce NF-κB, ISRE and BoIFNβ promotor activity on BT cells. … (more)
- Is Part Of:
- Developmental and comparative immunology. Volume 78(2018)
- Journal:
- Developmental and comparative immunology
- Issue:
- Volume 78(2018)
- Issue Display:
- Volume 78, Issue 2018 (2018)
- Year:
- 2018
- Volume:
- 78
- Issue:
- 2018
- Issue Sort Value:
- 2018-0078-2018-0000
- Page Start:
- 91
- Page End:
- 99
- Publication Date:
- 2018-01
- Subjects:
- Rabbit interferon-α -- Type I IFN locus -- Molecular characteristics -- Expression distribution -- Biological activity
Immunology -- Periodicals
Developmental immunology -- Periodicals
616.079 - Journal URLs:
- http://www.sciencedirect.com/science/journal/0145305X ↗
http://www.elsevier.com/journals ↗ - DOI:
- 10.1016/j.dci.2017.09.016 ↗
- Languages:
- English
- ISSNs:
- 0145-305X
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 3579.051000
British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 4804.xml