Genome wide conditional mouse knockout resources. (2016)

• Record Type: Journal Article
• Authors: Kaloff, C.; Anastassiadis, K.; Ayadi, A.; Baldock, R.; Beig, J.; Birling, M.-C.; Bradley, A.; Brown, S.D.M.; Bürger, A.; Bushell, W.; Chiani, F.; Collins, F.S.; Doe, B.; Eppig, J.T.; Finnell, R.H.; Fletcher, C.; Flicek, P.; Fray, M.; Friedel, R.H.; Gambadoro, A.; Gates, H.; Hansen, J.; Herault, Y.; Hicks, G.G.; Hörlein, A.; Hrabé de Angelis, M.; Iyer, V.; de Jong, P.J.; Koscielny, G.; Kühn, R.; Liu, P.; Lloyd, K.C.K; Lopez, R.G.; Marschall, S.; Martínez, S.; McKerlie, C.; Meehan, T.; von Melchner, H.; Moore, M.; Murray, S.A.; Nagy, A.; Nutter, L.M.J.; Pavlovic, G.; Pombero, A.; Prosser, H.; Ramirez-Solis, R.; Ringwald, M.; Rosen, B.; Rosenthal, N.; Rossant, J.; Ruiz Noppinger, P.; Ryder, E.; Skarnes, W.C.; Schick, J.; Schnütgen, F.; Schofield, P.; Seisenberger, C.; Selloum, M.; Smedley, D.; Simpson, E.M.; Stewart, A.F.; Teboul, L.; Tocchini Valentini, G.P.; Valenzuela, D.; West, A.P.; Wurst, W.;
• Is Part Of: Drug discovery today. Volume 20(2016)
• Page Start: 3
• Page End: 12
• Publication Date: 2016
• Subjects: Drug development -- Periodicals; Physiology, Pathological -- Periodicals; Drugs -- Testing -- Periodicals; Diseases -- Animal models -- Evaluation -- Periodicals; 616.027
• DOI: 10.1016/j.ddmod.2017.08.002
• Languages: English
• ISSNs: 1740-6757

Abstract

Abstract : The International Knockout Mouse Consortium (IKMC) developed high throughput gene trapping and gene targeting pipelines that produced mostly conditional mutations of more than 18, 500 genes in C57BL/6N mouse embryonic stem (ES) cells which have been archived and are freely available to the research community as a frozen resource. From this unprecedented resource more than 6000 mutant mouse strains have been generated by the IKMC in collaboration with the International Mouse Phenotyping Consortium (IMPC). In addition, a cre-driver resource was established including 250 C57BL/6 cre-inducible mouse strains. Complementing the cre-driver resource, a collection comprising 27 rAAVs expressing cre in a tissue-specific manner has also been produced. All resources are easily accessible from the IKMC/IMPC web portal (www.mousephenotype.org ). The IKMC/IMPC resource is a standardized reference library of mouse models with defined genetic backgrounds enabling the analysis of gene-disease associations in mice of different genetic makeup and should therefore have a major impact on biomedical research.