H19 non-coding RNA in urine cells detects urothelial carcinoma: a pilot study. (3rd October 2017)

• Record Type: Journal Article
• Authors: Gielchinsky, Ilan; Gilon, Michal; Abu-lail, Rasha; Matouk, Imad; Hochberg, Avraham; Gofrit, Ofer N.; Ben-Dov, Iddo Z.
• Is Part Of: Biomarkers. Volume 22:Number 7(2017)
• Page Start: 661
• Page End: 666
• Publication Date: 2017-10-03
• Subjects: Non-coding RNA -- diagnostic biomarkers -- urine biomarkers -- bladder cancer; Biochemical markers -- Periodicals; 610.28
• DOI: 10.1080/1354750X.2016.1276625
• Languages: English
• ISSNs: 1354-750X

Abstract

Abstract: Context: Urothelial carcinoma (UC) is common and highly recurrent. Diagnosis and follow-up involve invasive cystoscopies. Objective: To evaluate H19 RNA in urine cells as diagnostic tool for UC. Materials and methods: RT-PCR analysis of urine samples from healthy volunteers and UC patients. Results: H19 RNA was unequivocally detected in the urine of 90.5% of patients and 25.9% of controls. H19 copies were three orders of magnitude higher in patients. Receiver operating characteristic analysis showed an area under the curve of 0.933. Conclusions: This pilot study shows that urinary cell H19 is a highly sensitive test for UC and pending verification could transform patient management.