Nanogap‐Rich Au Nanowire SERS Sensor for Ultrasensitive Telomerase Activity Detection: Application to Gastric and Breast Cancer Tissues Diagnosis. (2nd August 2017)

• Record Type: Journal Article
• Authors: Eom, Gayoung; Kim, Hongki; Hwang, Ahreum; Son, Hye‐Young; Choi, Yuna; Moon, Jeong; Kim, Donghyeong; Lee, Miyeon; Lim, Eun‐Kyung; Jeong, Jinyoung; Huh, Yong‐Min; Seo, Min‐Kyo; Kang, Taejoon; Kim, Bongsoo
• Is Part Of: Advanced functional materials. Volume 27:Number 37(2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-08-02
• Subjects: gold nanowires -- nanogaps -- sensors -- surface‐enhanced Raman scattering -- telomerase activity; Materials -- Periodicals; Chemical vapor deposition -- Periodicals; 620.11
• DOI: 10.1002/adfm.201701832
• Languages: English
• ISSNs: 1616-301X

Abstract

Abstract : Telomerase has attracted much attention as a universal cancer biomarker because telomerase is overexpressed in more than 85% of human cancer cells while suppressed in normal somatic cells. Since a strong association exists between telomerase activity and human cancers, the development of effective telomerase activity assay is critically important. Here, a nanogap‐rich Au nanowire (NW) surface‐enhanced Raman scattering (SERS) sensor is reported for detection of telomerase activity in various cancer cells and tissues. The nanogap‐rich Au NWs are constructed by deposition of nanoparticles on single‐crystalline Au NWs and provided highly reproducible SERS spectra. The telomeric substrate (TS) primer‐attached nanogap‐rich Au NWs can detect telomerase activity through SERS measurement after the elongation of TS primers, folding into G‐quadruplex structures, and intercalation of methylene blue. This sensor enables us to detect telomerase activity from various cancer cell lines with a detection limit of 0.2 cancer cells mL −1 . Importantly, the nanogap‐rich Au NW sensor can diagnose gastric and breast cancer tissues accurately. The nanogap‐rich Au NW sensors show strong SERS signals only in the presence of tumor tissues excised from 16 tumor‐bearing mice, while negligible signals in the presence of heated tumor tissues or normal tissues. It is anticipated that nanogap‐rich Au NW SERS sensors can be used for a universal cancer diagnosis and further biomedical applications including a diverse biomarker sensing. Abstract : A nanogap‐rich Au nanowire (NW) surface‐enhanced Raman scattering (SERS) sensor is developed for ultrasensitive and specific detection of telomerase activity. The optimized nanogap‐rich Au NW can provide highly reproducible and strong SERS signals. Using nanogap‐rich Au NW SERS sensors, telomerase activities of cancer cells and tumor tissues are successfully detected, suggesting the potential applicability of nanogap‐rich Au NW SERS sensor in cancer diagnosis.