[PP.30.01] MICRO-92B SUPPRESS HAND2 TO MEDIATE ANGIOTENSIN-II-INDUCED CARDIAC HYPERTROPHY. (September 2017)

• Record Type: Journal Article
• Authors: Yu, X.; Huang, Y.; Hu, Z.; Shan, Z.; Feng, Y.
• Is Part Of: Journal of hypertension. Volume 35(2017)Supplement 2
• Publication Date: 2017-09
• Subjects: Hypertension -- Periodicals; Hypertension -- Periodicals; 616.132005
• DOI: 10.1097/01.hjh.0000523985.97047.57
• Languages: English
• ISSNs: 1473-5598

Abstract

Abstract : Objective: To investigate the expression and potential target of micro-92b (miR-92b) in angiotensin II (Ang-II)- induced mouse cardiac hypertrophy. Figure. No caption available. Design and method: We established mouse cardiac hypertrophy models of Ang-II infusion and pressure-overloading by transverse aortic constriction (TAC). To investigate the effect of miR-92b on Ang-II-induced hypertrophy in vivo, 24 C57BL/6 mice were randomized into 4 groups: (1) agomiR-negative control (NC) + saline, (2) agomiR-NC + Ang-II (NC agomir with Ang-II infusion), (3) agomiR-92 + saline (miR-92b agomir with saline infusion), and (4) agomiR-92 + Ang-II (miR-92b agomir with Ang-II infusion). Left ventricular (LV) function variables were assessed by transthoracic echocardiography. Wheat germ agglutinin (WGA) staining was performed on mouse ventricular myocardium. Neonatal mouse ventricular cells (NMVCs) were isolated from the hearts of 1-3-d-old newborn C57BL6 mice. FITC-phalloidin staining was used on cultured NMVCs. The expression of miR-92b and hypertrophic gene mRNA were measured by Reverse-transcription quantitative PCR. The expression of protein were determined by western blot analysis. Dual luciferase assay was for HAND2 target identification. Results: MiR-92b significantly decreases in hypertrophic myocardium and cardiomyocytes. Over-expression of miR-92b can result in a reduction of the expression of genes related to myocardial hypertrophy and ameliorate the angiotensin II (Ang-II) -induced cardiac hypertrophy in mice. Our date shows that miR-92b negatively regulated Heart and neural crest derivatives-expressed 2 (HAND2) expression by directly targeting the untranslated region (UTR) of HAND2 mRNA. We have also found that both miR-92b mimic and HAND2 siRNA could resultfully inhibit Ang-II-induced hypertrophy in mouse cardiomyocytes. Conclusions: miR-92b modulates HAND2 during myocardial hypertrophy and overexpression of miR-92b performs as a protective factor against myocardial hypertrophy.