[PP.20.20] SODIUM CHLORIDE ALTERS DENDRITIC CELL POLARIZATION TOWARDS TH1 CELLS. (September 2017)

• Record Type: Journal Article
• Authors: Al-Hajj, S.; Lemoine, R.; Héraud, A.; Lefort, C.; Benoist, L.; Kazma, I.; Lamendour, L.; Deluce, N.; Kouba, S.; Hoarau, C.; Gatault, P.; Buchler, M.; Baron, C.; Halimi, J.
• Is Part Of: Journal of hypertension. Volume 35(2017)Supplement 2
• Publication Date: 2017-09
• Subjects: Hypertension -- Periodicals; Hypertension -- Periodicals; 616.132005
• DOI: 10.1097/01.hjh.0000523736.11534.89
• Languages: English
• ISSNs: 1473-5598

Abstract

Abstract : Objective: High salt diet is related to the development of hypertension, increases skin dendritic cell (DC) density as well as interstitial sodium concentration (up to 180 mol/L). Recent in vitro studies indicated that high sodium concentrations polarize the T-cell immune response toward TH17. DC play a major role in innate and adaptive immune system. Interestingly, hypertension was recently shown to be transferable to normotensive animals through infusion of DC from hypertensive animals. However, the effects of high sodium concentration on DC function remain unknown. Design and method: In this in vitro study, we analyzed how high NaCl concentration interferes with LPS-induced DC maturation. DC were derived from human blood monocytes that were differentiated for 6 days into DCs in RPMI 10% Bovine calf serum supplemented with GMCSF (66 ng/ml) and IL4 (25 ng/ml) then stimulated during 48 h with LPS 2.5 ng/ml at increasing concentrations of NaCl [140 160 180 200 Mm]. We analyzed cell viability by Annexin V and Propidium iodide staining and cell expression of CCR7, CD25, CD86 and CD83 by flow cytometry. We also studied secreted cytokines such as IL-12p70 by ELISA method. Results: DC viability was maintained until a concentration of [200 mM] NaCl (above, viability was markedly reduced to 5%). High NaCl concentration reduced the expression of maturation markers (CD25+ 91.3 ± 2.8 vs 79.6 ± 5.9%, CD86+: 92.5 ± 4.4 vs 77.2 ± 3.0%, CD83+: 91.8 ± 4.4 vs 77.2 ± 5.6% with significates differences p < 0.01) for 140 mM and 200 mM NaCl, respectively. Of note, the expression of CCR7 diminished from (47.4 ± 12.5 to 19.4 ± 12.2% with significates differences p < 0.01) for 140 mM and 200 mM. We could reveal by ELISA method a significant IL12-p70 cytokine decrease from (225 ± 94.5 to 65.5 ± 25.7 pg/ml with significates differences p < 0.05) for 140 mM and 200 mM NaCl, respectively. Conclusions: High NaCl concentration reduced maturation and migration capacity of human DC, decreases IL12 production thus altering the polarization pathway towards Th1 immune cells which may directly affect our immune response against viral infection. Whether DC submitted to high NaCl concentrations may implicate specific cellular signaling pathways remains unknown.