Identification of biphenyl 2, 3-dioxygenase and its catabolic role for phenazine degradation in Sphingobium yanoikuyae B1. (15th December 2017)

• Record Type: Journal Article
• Authors: Zhao, Qiang; Bilal, Muhammad; Yue, Shengjie; Hu, Hongbo; Wang, Wei; Zhang, Xuehong
• Is Part Of: Journal of environmental management. Volume 204:Part 1(2017)
• Page Start: 494
• Page End: 501
• Publication Date: 2017-12-15
• Subjects: Phenazine -- Sphingobium yanoikuyae B1 -- Biodegradation -- Biphenyl 2, 3-dioxygenase; Environmental policy -- Periodicals; Environmental management -- Periodicals; Environment -- Periodicals; Ecology -- Periodicals; 363.705
• DOI: 10.1016/j.jenvman.2017.09.027
• Languages: English
• ISSNs: 0301-4797

Abstract

Abstract: Phenazines are important nitrogen-containing secondary metabolites that display a range of biological functionalities. However, these compounds have shown lethal effects on humans and, the fate of phenazine in the ecosystem remains uncertain. In this study, we investigated that Sphingobium yanoikuyae B1 could utilize phenazine as a sole carbon source for growth. Intermediate produced during phenazine degradation was purified and identified as 1, 2-dihydrogen 1, 2-dihydroxy phenazine. Biphenyl 2, 3-dioxygenase was determined to be the initial dioxygenase for phenazine degradation through gene cloning and whole cell transformation techniques. Phenazine was converted to 1, 2-dihydrogen 1, 2-dihydroxy phenazine through hydrogenation and hydroxylation, which then transformed to 2-hydroxy phenazine through spontaneous dehydration. The bphA1fA2f, were evidenced to be the only genes encoding the initial dioxygenase for phenazine degradation. BphB (dihydrodiol dehydrogenase) and BphC (2, 3-dihydroxybiphenyl 1, 2-dioxygenase) did not exhibit any 1, 2-dihydrogen 1, 2-dihydroxy phenazine and 1, 2-dihydroxy phenazine degradation capability, suggesting no contribution in phenazine degradation. Phylogenetic analysis of the dioxygenases demonstrated enormous biodegradation potential in strain B1. In conclusion, this study opens up new possibilities in better understanding the phenazine degradation in the environment. Graphical abstract: Highlights: Identification of a novel three-component phenazine-1-carboxylic acid 1, 2-dioxygenase. Biphenyl 2, 3-dioxygenase as the initial dioxygenase for phenazine degradation. 1, 2-dihydrogen 1, 2-dihydroxy phenazine as the key intermediate in phenazine degradation. Dioxygenase displays an enormous phenazine degradation potential in Sphingobium yanoikuyae B1.