Expression of prosaposin and its receptors in the rat cerebellum after kainic acid injection. (June 2017)

• Record Type: Journal Article
• Authors: Li, Xuan; Nabeka, Hiroaki; Saito, Shouichiro; Shimokawa, Tetsuya; Khan, Md. Sakirul Islam; Yamamiya, Kimiko; Shan, Fengping; Gao, Huiling; Li, Cheng; Matsuda, Seiji
• Is Part Of: IBRO reports. Volume 2(2017)
• Page Start: 31
• Page End: 40
• Publication Date: 2017-06
• Subjects: Prosaposin -- GPR37 -- GPR37L1 -- Kainic acid -- Cerebellum -- Neurodegeneration; BSA bovine serum albumin -- ER endoplasmic reticulum -- GPCR G protein-coupled receptor -- H-E staining hematoxylin-eosin staining -- IF immunofluorescence -- IHC immunohistochemistry -- ISH in situ hybridization -- KA kainic acid -- PSAP prosaposin -- SSC standard saline citrate; Nervous system -- Periodicals; Brain -- Periodicals; Brain -- Research -- Periodicals; Nervous system; Brain -- Research; Brain; Neurology; Nervous System Physiological Phenomena; Electronic journals; Periodicals; Fulltext; Internet Resources; Periodicals; Periodical
• DOI: 10.1016/j.ibror.2017.02.002
• Languages: English
• ISSNs: 2451-8301

Abstract

Abstract: Prosaposin (PSAP), a highly conserved glycoprotein, is a precursor of saposins A–D. Accumulating evidence suggests that PSAP is a neurotrophic factor that induces differentiation and prevents death in a variety of neuronal cells through the active region within the saposin C domain both in vivo and in vitro . Recently, GPR37 and GPR37L1 were recognized as PSAP receptors. In this study, we examined the alteration in expression of PSAP and its receptors in the cerebellum using rats injected with kainic acid (KA). The results show that PSAP was strongly expressed in the cytoplasm of Purkinje cells and interneurons in the molecular layer, and that PSAP expression in both types of neurons was markedly enhanced following KA treatment. Immunoblotting revealed that the expression of GPR37 was diminished significantly three days after KA injection compared with control rats; however, no changes were observed through immunostaining. No discernable changes were found in GPR37L1. These findings may help us to understand the role of PSAP and the GPR37 and GPR37L1 receptors in alleviating the neural damage caused by KA.