Molecular signature and functional analysis of uterine ILCs in mouse pregnancy. (September 2017)

• Record Type: Journal Article
• Authors: Li, Minmin; Gao, Yimin; Yong, Liang; Huang, Dake; Shen, Jijia; Liu, Miao; Ren, Cuiping; Hou, Xin
• Is Part Of: Journal of reproductive immunology. Volume 123(2017)
• Page Start: 48
• Page End: 57
• Publication Date: 2017-09
• Subjects: ILCs innate lymphoid cells -- uILCs uterine ILCs -- Nfil3 transcription factor nuclear factor, interleukin 3 -- MNCs mononuclear cells -- i.p. intraperitoneally -- H&E hematoxylin/eosin -- MLAP mesometrial lymphoid aggregate of pregnancy -- gd gestation day -- trNK tissue-resident NK -- cNK conventional NK -- DB decidua basalis; Uterine -- Innate lymphoid cells -- Pregnancy -- Spiral artery remodeling; Reproduction -- Immunological aspects -- Periodicals; Immunology -- Periodicals; Allergy and Immunology -- Periodicals; Reproduction -- Periodicals; Reproduction -- Immunologie -- Périodiques; Immunologie -- Périodiques; Immunology; Reproduction -- Immunological aspects; Periodicals; Electronic journals; Electronic journals; 615.766
• DOI: 10.1016/j.jri.2017.09.003
• Languages: English
• ISSNs: 0165-0378

Abstract

Highlights: Uterine ILCs especially ILC2s increased markedly at early-gestation (gd6.5). Depletion of uterine ILCs in Rag1 −/− mice resulted in impaired uterine spiral artery remodeling at mid-gestation (gd10.5). uILC depletion did not alter pregnancy rate, fetal viability or fetal weight in late-gestation (gd16.5). Abstract: In addition to natural killer cells, other innate lymphoid cells have recently been identified in the mouse and human uterus, but their roles in successful pregnancy remain poorly defined. In this study, we examined the dynamic changes of uterine innate lymphoid cells throughout pregnancy in mice. We found that the total number of uterine innate lymphoid cells markedly increased at early-gestation. Among the three groups of uterine innate lymphoid cells, the number of the group 2 uterine innate lymphoid cells increased the most during pregnancy. We also determined that the depletion of uterine innate lymphoid cells in Rag1 −/− mice resulted in impaired uterine spiral artery remodeling. These results suggest that uterine innate lymphoid cells may play an important role in mouse reproduction.