[BP.04.06] CIRCULATING ENDOTHELIAL-DERIVED APOPTOTIC MICROPARTICLES TO MONONUCLEAR PROGENITOR CELLS RATIO AS A PREDICTOR OF THROMBOEMBOLIC EVENTS IN PATIENTS WITH ACUTELY DECOMPENSATED HEART. (September 2017)

• Record Type: Journal Article
• Authors: Berezin, A.; Kremzer, A.; Berezina, T.
• Is Part Of: Journal of hypertension. Volume 35(2017)Supplement 2
• Publication Date: 2017-09
• Subjects: Hypertension -- Periodicals; Hypertension -- Periodicals; 616.132005
• DOI: 10.1097/01.hjh.0000523490.57642.16
• Languages: English
• ISSNs: 1473-5598

Abstract

Abstract : Objective: The objective of the study was to examine prognostic value of circulating endothelial-derived apoptotic microparticles (EMPs) to endothelial origin mononuclear progenitor cells (MPCs) ratio for post-discharged patients after acutely decompensated heart failure (ADHF). Design and method: We have been consecutively enrolled 118 clinically stable HF patients (72 male) admitted with a primary diagnosis of ADHF. All the patients have given their written informed consent for participation in the study. At baseline all enrolled patients were hemodynamically stable and they had NYHA III/IV classes of chronic HF. Observation period was up to 3 years. Flow cytometry analysis for quantifying the number of EMPs and angiogenic MPCs was used. The flow cytometric technique was used for predictably distinguishing circulating cell subsets depending on expression of CD45, CD34, CD14, Tie-2, and CD309 antigens and determining endothelial cell-derived microparticles. CD31+/annexin V+ was defined as apoptotic endothelial cell-derived MPs, MPs labeled for CD105+ or CD62E+ were determined as MPs produced due to activation of endothelial cells. Results: Thirty six thromboembolic events (TEE) were determined within 3 years. Calculated EMPs to MPCs ratios in patients without and with TEE were 7.9 (95% CI = 7.4 – 9.0) and 66.5 (95% CI = 47.0 – 110.4) respectively (P = 0.001). MPCs to EMPs ration, NYHA class, galectin-3 and NT-pro-BNP at discharge, increased NT-pro-BNP > 30% within hospitalization period were found as independent predictors of TTE rate. All these predictors were compounded into predictive Model 1. However, adding of EPMs to MPCs ratio to the Model 1 has improved the relative IDI by 17.4% for TTE rate, by 20.1% for fatal TTE, and by 18.1% for TTE-related re-admission. Conclusions: We demonstrated that EMPs to MPCs ratio might considered a tremendous predictor of TTE and HF-related outcomes in post-discharged clinically stabilized HF patients.