Autophagy inhibition of immature oocytes during vitrification-warming and in vitro mature activates apoptosis via caspase-9 and −12 pathway. (October 2017)

• Record Type: Journal Article
• Authors: Gao, Hai-Hua; Li, Jun-Tao; Liu, Jing-Jing; Yang, Qing-Ai; Zhang, Jian-Min
• Is Part Of: European journal of obstetrics, gynecology, and reproductive biology. Volume 217(2017)
• Page Start: 89
• Page End: 93
• Publication Date: 2017-10
• Subjects: Autophagy -- Immature oocytes -- Vitrification -- Apoptosis -- Caspase; Obstetrics -- Periodicals; Gynecology -- Periodicals; Reproductive health -- Periodicals; Gynecology -- Periodicals; Obstetrics -- Periodicals; Reproduction -- Periodicals; Obstétrique -- Périodiques; Gynécologie -- Périodiques; Reproduction -- Périodiques; Verloskunde; Gynaecologie; Voortplanting (biologie); Gynecology; Obstetrics; Reproduction; Electronic journals; Periodicals; Electronic journals; 618.05
• DOI: 10.1016/j.ejogrb.2017.08.029
• Languages: English
• ISSNs: 0301-2115

Abstract

Abstract: Objective: The aim of this study is to determine the role of autophagy in the immature oocytes during vitrification-warming and in vitro maturation (IVM); the correlations among autophagy, apoptosis, and the activities of caspase in the immature oocytes during vitrification-warming and IVM were also explored. Study design: Immature oocytes from mice were vitrified-warmed and IVM. An autophagy inhibitor (3-methyladenine) was supplemented in cryopreservation solutions and warming solutions. The expression of beclin-1 (an autophagy marker), caspase-3, −8, −9, and −12 were measured. Moreover, the viability of vitrified-warmed immature oocytes and their subsequent developmental competence were measured. Results: The levels of beclin-1 expression in both mRNA and protein in oocytes experienced vitrification-warming and IVM were significantly higher than that in fresh immature oocytes experienced IVM. The levels of caspase-3, −9, −12 expression in both mRNA and protein in oocytes vitrified with 3-methyladenine were significantly higher than that vitrified without 3-methyladenine. However, the differences in the caspase-8 expression in both mRNA and protein between the oocytes vitrified with 3-methyladenine and that vitrified without 3-methyladenine were not significant. Conclusion: Immature oocyte cryopreservation exhibits autophagic activation. Autophagy inhibition of the immature oocytes during vitrification-warming and IVM activates apoptosis via caspase-9 and –12 pathway.