Evaluation of the routine antimicrobial susceptibility testing results of clinically significant anaerobic bacteria in a Slovenian tertiary-care hospital in 2015. (October 2017)

• Record Type: Journal Article
• Authors: Jeverica, Samo; Kolenc, Urša; Mueller-Premru, Manica; Papst, Lea
• Is Part Of: Anaerobe. Volume 47(2017)
• Page Start: 64
• Page End: 69
• Publication Date: 2017-10
• Subjects: Anaerobic bacteria -- Antimicrobial susceptibility testing -- Antimicrobial resistance -- Surveillance -- Slovenia; Anaerobic infections -- Periodicals; Anaerobic bacteria -- Periodicals; Bacterial diseases -- Periodicals; Computer network resources; Anaerobic protozoa -- Periodicals; 579.3
• DOI: 10.1016/j.anaerobe.2017.04.007
• Languages: English
• ISSNs: 1075-9964

Abstract

Abstract: The aim of our study was to determined antimicrobial susceptibility profiles of 2673 clinically significant anaerobic bacteria belonging to the major genera, isolated in 2015 in a large tertiary-care hospital in Slovenia. The species identification was performed by MALDI-TOF mass spectrometry. Antimicrobial susceptibility was determined immediately at the isolation of the strains against: penicillin, co-amoxiclav, imipenem, clindamycin and metronidazole, using gradient diffusion methodology and EUCAST breakpoints. The most frequent anaerobes were Bacteroides fragilis group with 31% (n = 817), Gram positive anaerobic cocci (GPACs) with 22% (n = 589), Prevotella with 14% (n = 313) and Propionibacterium with 8% (n = 225). Metronidazole has retained full activity (100%) against all groups of anaerobic bacteria intrinsically susceptible to it. Co-amoxiclav and imipenem were active against most tested anaerobes with zero or low resistance rates. However, observed resistance to co-amoxiclav (8%) and imipenem (1%) is worrying especially among B. fragilis group isolates. High overall resistance (23%) to clindamycin was detected in our study and was highest among the genera Prevotella, Bacteroides, Parabacteroides, GPACs and Clostridium . Routine testing of antimicrobial susceptibility of clinically relevant anaerobic bacteria is feasible and provides good surveillance data. Highlights: 2673 clinically relevant anaerobes were tested for resistance to 5 antimicrobials. Metronidazole was active against all anaerobes intrinsically susceptible to it. Resistance to co-amoxiclav among B. fragilis group was 8%. High overall resistance (23%) to clindamycin among all anaerobes is worrying.