A novel antimicrobial peptide against dental-caries-associated bacteria. (October 2017)

• Record Type: Journal Article
• Authors: Chen, Long; Jia, Lili; Zhang, Qiang; Zhou, Xirui; Liu, Zhuqing; Li, Bingjie; Zhu, Zhentai; Wang, Fenwei; Yu, Changyuan; Zhang, Qian; Chen, Feng; Luo, Shi-Zhong
• Is Part Of: Anaerobe. Volume 47(2017)
• Page Start: 165
• Page End: 172
• Publication Date: 2017-10
• Subjects: Antimicrobial peptides -- Streptococcus mutans -- Dental caries -- Biofilm -- Oral bacteria; Anaerobic infections -- Periodicals; Anaerobic bacteria -- Periodicals; Bacterial diseases -- Periodicals; Computer network resources; Anaerobic protozoa -- Periodicals; 579.3
• DOI: 10.1016/j.anaerobe.2017.05.016
• Languages: English
• ISSNs: 1075-9964

Abstract

Abstract: Dental caries, a highly prevalent oral disease, is primarily caused by pathogenic bacteria infection, and most of them are anaerobic. Herein, we investigated the activity of a designed antimicrobial peptide ZXR-2, and found it showed broad-spectrum activity against a variety of Gram-positive and Gram-negative oral bacteria, particularly the caries-related taxa Streptococcus mutans . Time-course killing assays indicated that ZXR-2 killed most bacterial cells within 5 min at 4 × MIC. The mechanism of ZXR-2 involved disruption of cell membranes, as observed by scanning electron microscopy. Moreover, ZXR-2 inhibited the formation of S. mutans biofilm, but showed limited hemolytic effect. Based on its potent antimicrobial activity, rapid killing, and inhibition of S. mutans biofilm formation, ZXR-2 represents a potential therapeutic for the prevention and treatment of dental caries. Highlights: The activity of AMP ZXR-2 against oral facultative anaerobic bacteria was tested. ZXR-2 showed fast-killing activity and limited hemolytic activity. ZXR-2 prevented the formation of S. mutans biofilms.